caa a22 4500 605503273 CHVBK 20210128100610.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00253-015-6739-2 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6739-2 Molecular methods for studying methanogens of the human gastrointestinal tract: current status and future directions [Elektronische Daten] [Prem Chaudhary, Nadia Gaci, Guillaume Borrel, Paul O'Toole, Jean-François Brugère] Until recently, human gut microbiota was believed to be colonized by few methanogenic archaeal species. Much higher microbial diversity within the human gut was revealed by the use of molecular approaches as compared to routine microbiological techniques, but still, a lot remains unknown. Molecular techniques has the advantage of being rapid, reproducible, and can be highly discriminative as compared to conventional culturing methods. Some of them provide both qualitative and quantitative information. However, the choice of method should be taken with care to avoid biases. The advent of next-generation sequencing gives much deeper information from which functional and ecological hypotheses can be drawn. In this review, molecular techniques that are currently used together with their possible future developments to study gut methanogenic communities are indicated along with their limitations and difficulties that are encountered during their implementation. Moreover, the high amount of metagenomics data from the human gut microbiome indicate that this environment could be a paradigm for new directions in methanogen diversity studies and help to develop new approaches for other environments as well. Concerning humans, this should help us to better understand the possible association of methanogens with some of the diseased conditions and their peculiar distribution among age groups in human. Springer-Verlag Berlin Heidelberg, 2015 Methanogens nationallicence Diversity nationallicence Molecular tools nationallicence Human gastrointestinal tract nationallicence Molecular marker nationallicence Chaudhary Prem EA 4678 CIDAM, Clermont Université; Université d'Auvergne, Clermont-Ferrand, France aut Gaci Nadia EA 4678 CIDAM, Clermont Université; Université d'Auvergne, Clermont-Ferrand, France aut Borrel Guillaume Department of Microbiology and Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland aut O'Toole Paul Department of Microbiology and Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland aut Brugère Jean-François EA 4678 CIDAM, Clermont Université; Université d'Auvergne, Clermont-Ferrand, France aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/14(2015-07-01), 5801-5815 0175-7598 99:14<5801 2015 99 253 https://doi.org/10.1007/s00253-015-6739-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 review-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6739-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chaudhary Prem EA 4678 CIDAM, Clermont Université; Université d'Auvergne, Clermont-Ferrand, France aut NATIONALLICENCE 700 1- Gaci Nadia EA 4678 CIDAM, Clermont Université; Université d'Auvergne, Clermont-Ferrand, France aut NATIONALLICENCE 700 1- Borrel Guillaume Department of Microbiology and Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland aut NATIONALLICENCE 700 1- O'Toole Paul Department of Microbiology and Alimentary Pharmabiotic Centre, University College Cork, Cork, Ireland aut NATIONALLICENCE 700 1- Brugère Jean-François EA 4678 CIDAM, Clermont Université; Université d'Auvergne, Clermont-Ferrand, France aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/14(2015-07-01), 5801-5815 0175-7598 99:14<5801 2015 99 253