caa a22 4500 605503303 CHVBK 20210128100610.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00253-015-6493-5 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6493-5 Archaeal community diversity in municipal waste landfill sites [Elektronische Daten] [Liyan Song, Yangqing Wang, Wei Tang, Yu Lei] Despite the pivotal role of archaea in methane production in landfills, the identity, ecology, and functional diversity of these microorganisms and their link to environmental factors remain largely unknown. We collected 11 landfill leachate samples from six geographically distinct landfills of different ages in China and analyzed the archaeal community by bar-coded 454 pyrosequencing. We retrieved 121,797 sequences from a total of 167,583 sequences (average length of 464bp). The archaeal community was geographically structured, and nonabundant taxa primarily contributed to the observed dissimilarities. Canonical correlation analysis (CCA) suggested that the total phosphorous (TP), nitrate, and conductivity are important drivers for shaping the archaeal community. The hydrogenotrophic methanogens Methanomicrobiales and Methanobacteriales greatly dominated 9 of 11 samples, ranging from 83.7 to 99.5%. These methanogens also dominated the remaining two samples, accounting for 70.3 and 58.8%, respectively. Interestingly, for all of the studied Chinese landfills, 16S rRNA analysis indicated the predominance of hydrogenotrophic methanogens. Springer-Verlag Berlin Heidelberg, 2015 Landfill nationallicence Landfill leachate nationallicence Archaeal community nationallicence Hydrogenotrophic methanogenesis nationallicence Song Liyan Research Center of Environmental Microbiology and Ecology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Science, No 266 Fangzhen Avenue, Shuitu High-Tech Park, 400714, Chongqing, Beibei, China aut Wang Yangqing Research Center of Environmental Microbiology and Ecology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Science, No 266 Fangzhen Avenue, Shuitu High-Tech Park, 400714, Chongqing, Beibei, China aut Tang Wei Research Center of Environmental Microbiology and Ecology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Science, No 266 Fangzhen Avenue, Shuitu High-Tech Park, 400714, Chongqing, Beibei, China aut Lei Yu Research Center of Environmental Microbiology and Ecology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Science, No 266 Fangzhen Avenue, Shuitu High-Tech Park, 400714, Chongqing, Beibei, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/14(2015-07-01), 6125-6137 0175-7598 99:14<6125 2015 99 253 https://doi.org/10.1007/s00253-015-6493-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6493-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Song Liyan Research Center of Environmental Microbiology and Ecology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Science, No 266 Fangzhen Avenue, Shuitu High-Tech Park, 400714, Chongqing, Beibei, China aut NATIONALLICENCE 700 1- Wang Yangqing Research Center of Environmental Microbiology and Ecology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Science, No 266 Fangzhen Avenue, Shuitu High-Tech Park, 400714, Chongqing, Beibei, China aut NATIONALLICENCE 700 1- Tang Wei Research Center of Environmental Microbiology and Ecology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Science, No 266 Fangzhen Avenue, Shuitu High-Tech Park, 400714, Chongqing, Beibei, China aut NATIONALLICENCE 700 1- Lei Yu Research Center of Environmental Microbiology and Ecology, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Science, No 266 Fangzhen Avenue, Shuitu High-Tech Park, 400714, Chongqing, Beibei, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/14(2015-07-01), 6125-6137 0175-7598 99:14<6125 2015 99 253