A new salicylate synthase AmS is identified for siderophores biosynthesis in Amycolatopsis methanolica 239T
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| 024 | 7 | 0 | |a 10.1007/s00253-014-6370-7 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-014-6370-7 | ||
| 245 | 0 | 2 | |a A new salicylate synthase AmS is identified for siderophores biosynthesis in Amycolatopsis methanolica 239T |h [Elektronische Daten] |c [Feng Xie, Shengwang Dai, Jinzhao Shen, Biao Ren, Pei Huang, Qiushui Wang, Xueting Liu, Buchang Zhang, Huanqin Dai, Lixin Zhang] |
| 520 | 3 | |a Siderophores are important for the growth of bacteria or the applications in treatment of iron overload-associated diseases due to the iron-chelating property. Salicylate synthase played a key role in the biosynthesis of some NRPS-derived siderophores by the providing of an iron coordination moiety as the initial building block. A new salicylate synthase, namely AmS, was identified in the biosynthesis pathway of siderophore amychelin in Amycolatopsis methanolica 239T, since it shunt chorismate, an integrant precursor, from primary to secondary metabolite flow. The amino acid sequence alignment and phylogenetic analysis showed that AmS grouped into a new cluster. In vitro assays of AmS revealed its wide temperature tolerance ranged from 0 to 40°C and narrow pH tolerant ranged from 7.0 to 9.0. AmS was resistant to organic solvents and non-ionic detergents. Moreover, AmS converted chorismate to salicylate with K m of 129.05μM, k cat of 2.20min−1 at optimal conditions, indicating its low substrate specificity and comparable velocity to reported counterparts (Irp9 and MbtI). These properties of AmS may improve the iron-seizing ability of A. methanolica to compete with its neighbors growing in natural environments. Most importantly, serine and cysteine residues were found to be important for the catalytic activity of AmS. This study presented AmS as a new cluster of salicylate synthase and the reaction mechanism and potential applications of salicylate synthase were highlighted as well. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Siderophore |2 nationallicence | |
| 690 | 7 | |a Salicylate synthase |2 nationallicence | |
| 690 | 7 | |a Drug target |2 nationallicence | |
| 690 | 7 | |a Biochemical properties |2 nationallicence | |
| 690 | 7 | |a Amycolatopsis |2 nationallicence | |
| 700 | 1 | |a Xie |D Feng |u School of Life Sciences, University of Science and Technology of China, No. 443 HuangShan Road, 230061, Hefei, China |4 aut | |
| 700 | 1 | |a Dai |D Shengwang |u CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, No. 1 Beichen West Road, Chaoyang District, 100101, Beijing, China |4 aut | |
| 700 | 1 | |a Shen |D Jinzhao |u CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, No. 1 Beichen West Road, Chaoyang District, 100101, Beijing, China |4 aut | |
| 700 | 1 | |a Ren |D Biao |u CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, No. 1 Beichen West Road, Chaoyang District, 100101, Beijing, China |4 aut | |
| 700 | 1 | |a Huang |D Pei |u CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, No. 1 Beichen West Road, Chaoyang District, 100101, Beijing, China |4 aut | |
| 700 | 1 | |a Wang |D Qiushui |u CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, No. 1 Beichen West Road, Chaoyang District, 100101, Beijing, China |4 aut | |
| 700 | 1 | |a Liu |D Xueting |u CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, No. 1 Beichen West Road, Chaoyang District, 100101, Beijing, China |4 aut | |
| 700 | 1 | |a Zhang |D Buchang |u Institute of Health Sciences, Anhui University, No. 111 Jiulong Road, 230601, Hefei, China |4 aut | |
| 700 | 1 | |a Dai |D Huanqin |u CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, No. 1 Beichen West Road, Chaoyang District, 100101, Beijing, China |4 aut | |
| 700 | 1 | |a Zhang |D Lixin |u School of Life Sciences, University of Science and Technology of China, No. 443 HuangShan Road, 230061, Hefei, China |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/14(2015-07-01), 5895-5905 |x 0175-7598 |q 99:14<5895 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-014-6370-7 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-014-6370-7 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Xie |D Feng |u School of Life Sciences, University of Science and Technology of China, No. 443 HuangShan Road, 230061, Hefei, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Dai |D Shengwang |u CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, No. 1 Beichen West Road, Chaoyang District, 100101, Beijing, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Shen |D Jinzhao |u CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, No. 1 Beichen West Road, Chaoyang District, 100101, Beijing, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ren |D Biao |u CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, No. 1 Beichen West Road, Chaoyang District, 100101, Beijing, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Huang |D Pei |u CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, No. 1 Beichen West Road, Chaoyang District, 100101, Beijing, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Wang |D Qiushui |u CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, No. 1 Beichen West Road, Chaoyang District, 100101, Beijing, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Liu |D Xueting |u CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, No. 1 Beichen West Road, Chaoyang District, 100101, Beijing, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhang |D Buchang |u Institute of Health Sciences, Anhui University, No. 111 Jiulong Road, 230601, Hefei, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Dai |D Huanqin |u CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, No. 1 Beichen West Road, Chaoyang District, 100101, Beijing, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhang |D Lixin |u School of Life Sciences, University of Science and Technology of China, No. 443 HuangShan Road, 230061, Hefei, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/14(2015-07-01), 5895-5905 |x 0175-7598 |q 99:14<5895 |1 2015 |2 99 |o 253 | ||