Metabolic engineering of Clostridium tyrobutyricum for n -butanol production from maltose and soluble starch by overexpressing α-glucosidase
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| 024 | 7 | 0 | |a 10.1007/s00253-015-6680-4 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6680-4 | ||
| 245 | 0 | 0 | |a Metabolic engineering of Clostridium tyrobutyricum for n -butanol production from maltose and soluble starch by overexpressing α-glucosidase |h [Elektronische Daten] |c [Le Yu, Mengmeng Xu, I-Ching Tang, Shang-Tian Yang] |
| 520 | 3 | |a Clostridium tyrobutyricum does not have the enzymes needed for using maltose or starch. Two extracellular α-glucosidases encoded by agluI and agluII from Clostridium acetobutylicum ATCC 824 catalyzing the hydrolysis of α-1,4-glycosidic bonds in maltose and starch from the non-reducing end were cloned and expressed in C. tyrobutyricum (Δack, adhE2), and their effects on n-butanol production from maltose and soluble starch in batch fermentations were studied. Compared to the parental strain grown on glucose, mutants expressing agluI showed robust activity in breaking down maltose and produced more butanol (17.2 vs. 9.5g/L) with a higher butanol yield (0.20 vs. 0.10g/g) and productivity (0.29 vs. 0.16g/Lh). The mutant was also able to use soluble starch as substrate, although at a slower rate compared to maltose. Compared to C. acetobutylicum ATCC 824, the mutant produced more butanol from maltose (17.2 vs. 11.2g/L) and soluble starch (16.2 vs. 8.8g/L) in batch fermentations. The mutant was stable in batch fermentation without adding antibiotics, achieving a high butanol productivity of 0.40g/Lh. This mutant strain thus can be used in industrial production of n-butanol from maltose and soluble starch. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a α-Glucosidase |2 nationallicence | |
| 690 | 7 | |a Butanol |2 nationallicence | |
| 690 | 7 | |a Clostridium tyrobutyricum |2 nationallicence | |
| 690 | 7 | |a Maltose |2 nationallicence | |
| 690 | 7 | |a Metabolic engineering |2 nationallicence | |
| 690 | 7 | |a Soluble starch |2 nationallicence | |
| 700 | 1 | |a Yu |D Le |u William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave, 43210, Columbus, OH, USA |4 aut | |
| 700 | 1 | |a Xu |D Mengmeng |u William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave, 43210, Columbus, OH, USA |4 aut | |
| 700 | 1 | |a Tang |D I-Ching |u Bioprocessing Innovative Company, 4734 Bridle Path Ct, 43017, Dublin, OH, USA |4 aut | |
| 700 | 1 | |a Yang |D Shang-Tian |u William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave, 43210, Columbus, OH, USA |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/14(2015-07-01), 6155-6165 |x 0175-7598 |q 99:14<6155 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6680-4 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6680-4 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Yu |D Le |u William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave, 43210, Columbus, OH, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Xu |D Mengmeng |u William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave, 43210, Columbus, OH, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Tang |D I-Ching |u Bioprocessing Innovative Company, 4734 Bridle Path Ct, 43017, Dublin, OH, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Yang |D Shang-Tian |u William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave, 43210, Columbus, OH, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/14(2015-07-01), 6155-6165 |x 0175-7598 |q 99:14<6155 |1 2015 |2 99 |o 253 | ||