caa a22 4500 605503354 CHVBK 20210128100610.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00253-015-6680-4 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6680-4 Metabolic engineering of Clostridium tyrobutyricum for n -butanol production from maltose and soluble starch by overexpressing α-glucosidase [Elektronische Daten] [Le Yu, Mengmeng Xu, I-Ching Tang, Shang-Tian Yang] Clostridium tyrobutyricum does not have the enzymes needed for using maltose or starch. Two extracellular α-glucosidases encoded by agluI and agluII from Clostridium acetobutylicum ATCC 824 catalyzing the hydrolysis of α-1,4-glycosidic bonds in maltose and starch from the non-reducing end were cloned and expressed in C. tyrobutyricum (Δack, adhE2), and their effects on n-butanol production from maltose and soluble starch in batch fermentations were studied. Compared to the parental strain grown on glucose, mutants expressing agluI showed robust activity in breaking down maltose and produced more butanol (17.2 vs. 9.5g/L) with a higher butanol yield (0.20 vs. 0.10g/g) and productivity (0.29 vs. 0.16g/Lh). The mutant was also able to use soluble starch as substrate, although at a slower rate compared to maltose. Compared to C. acetobutylicum ATCC 824, the mutant produced more butanol from maltose (17.2 vs. 11.2g/L) and soluble starch (16.2 vs. 8.8g/L) in batch fermentations. The mutant was stable in batch fermentation without adding antibiotics, achieving a high butanol productivity of 0.40g/Lh. This mutant strain thus can be used in industrial production of n-butanol from maltose and soluble starch. Springer-Verlag Berlin Heidelberg, 2015 α-Glucosidase nationallicence Butanol nationallicence Clostridium tyrobutyricum nationallicence Maltose nationallicence Metabolic engineering nationallicence Soluble starch nationallicence Yu Le William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave, 43210, Columbus, OH, USA aut Xu Mengmeng William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave, 43210, Columbus, OH, USA aut Tang I-Ching Bioprocessing Innovative Company, 4734 Bridle Path Ct, 43017, Dublin, OH, USA aut Yang Shang-Tian William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave, 43210, Columbus, OH, USA aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/14(2015-07-01), 6155-6165 0175-7598 99:14<6155 2015 99 253 https://doi.org/10.1007/s00253-015-6680-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6680-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yu Le William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave, 43210, Columbus, OH, USA aut NATIONALLICENCE 700 1- Xu Mengmeng William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave, 43210, Columbus, OH, USA aut NATIONALLICENCE 700 1- Tang I-Ching Bioprocessing Innovative Company, 4734 Bridle Path Ct, 43017, Dublin, OH, USA aut NATIONALLICENCE 700 1- Yang Shang-Tian William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Ave, 43210, Columbus, OH, USA aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/14(2015-07-01), 6155-6165 0175-7598 99:14<6155 2015 99 253