caa a22 4500 605503362 CHVBK 20210128100610.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00253-015-6603-4 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6603-4 Bioprospecting of microalgae for integrated biomass production and phytoremediation of unsterilized wastewater and anaerobic digestion centrate [Elektronische Daten] [Pavlo Bohutskyi, Kexin Liu, Laila Nasr, Natalie Byers, Julian Rosenberg, George Oyler, Michael Betenbaugh, Edward Bouwer] Eighteen microalgae, including two local isolates, were evaluated for their ability to grow and remove nutrients from unsterilized primary or secondary wastewater effluents as well as wastewater supplemented with nutrient-rich anaerobic digester centrate (ADC). Most of the tested species except several phylogenetically clustered Chlorella sorokiniana including local isolates and Scenedesmus strains were unable to grow efficiently. This may reflect the presence of certain genetic traits important for robust growth in the unsterilized wastewater. The maximum algal-specific growth rates and biomass density obtained in these bacterial-contaminated cultures were in the range of 0.8-1day−1 and 250-350mgL−1, respectively. ADC supplementation was especially helpful to biologically treated secondary effluent with its lower initial macronutrient and micronutrient content. As a result of algal growth, total nitrogen and orthophosphate levels were reduced by as much as 90 and 70%, respectively. Biological assimilation was estimated to be the main mechanism of nitrogen removal in primary and secondary effluents with ammonia volatilization and bacterial nitrification-denitrification contributing for cultures supplemented with ADC. Assimilation by algae served as the principal mechanism of orthophosphate remediation in secondary wastewater cultures, while chemical precipitation appeared also to be important for orthophosphate removal in primary wastewater. Overall, cultivation of microalgae in primary and primary + 5% ADC may be more favorable from an economical and sustainability perspective due to elimination of the costly and energy-intensive biological treatment step. These findings demonstrate that unsterilized wastewater and ADC can serve as critical nutrient sources for biomass generation and that robust microalgae can be potent players in wastewater phytoremediation. Springer-Verlag Berlin Heidelberg, 2015 Microalgal cultivation nationallicence Wastewater treatment nationallicence Nutrient removal nationallicence Anaerobic digester effluent nationallicence Indigenous algal species nationallicence Phylogenetic analysis nationallicence Bohutskyi Pavlo Department of Geography & Environmental Engineering, Johns Hopkins University, 3400 North Charles Street, 21218-2686, Baltimore, MD, USA aut Liu Kexin Department of Geography & Environmental Engineering, Johns Hopkins University, 3400 North Charles Street, 21218-2686, Baltimore, MD, USA aut Nasr Laila Department of Geography & Environmental Engineering, Johns Hopkins University, 3400 North Charles Street, 21218-2686, Baltimore, MD, USA aut Byers Natalie Department of Geography & Environmental Engineering, Johns Hopkins University, 3400 North Charles Street, 21218-2686, Baltimore, MD, USA aut Rosenberg Julian Department of Chemical & Biomolecular Engineering, Johns Hopkins University, 3400 North Charles Street, 21218-2686, Baltimore, MD, USA aut Oyler George Department of Chemical & Biomolecular Engineering, Johns Hopkins University, 3400 North Charles Street, 21218-2686, Baltimore, MD, USA aut Betenbaugh Michael Department of Chemical & Biomolecular Engineering, Johns Hopkins University, 3400 North Charles Street, 21218-2686, Baltimore, MD, USA aut Bouwer Edward Department of Geography & Environmental Engineering, Johns Hopkins University, 3400 North Charles Street, 21218-2686, Baltimore, MD, USA aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/14(2015-07-01), 6139-6154 0175-7598 99:14<6139 2015 99 253 https://doi.org/10.1007/s00253-015-6603-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6603-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bohutskyi Pavlo Department of Geography & Environmental Engineering, Johns Hopkins University, 3400 North Charles Street, 21218-2686, Baltimore, MD, USA aut NATIONALLICENCE 700 1- Liu Kexin Department of Geography & Environmental Engineering, Johns Hopkins University, 3400 North Charles Street, 21218-2686, Baltimore, MD, USA aut NATIONALLICENCE 700 1- Nasr Laila Department of Geography & Environmental Engineering, Johns Hopkins University, 3400 North Charles Street, 21218-2686, Baltimore, MD, USA aut NATIONALLICENCE 700 1- Byers Natalie Department of Geography & Environmental Engineering, Johns Hopkins University, 3400 North Charles Street, 21218-2686, Baltimore, MD, USA aut NATIONALLICENCE 700 1- Rosenberg Julian Department of Chemical & Biomolecular Engineering, Johns Hopkins University, 3400 North Charles Street, 21218-2686, Baltimore, MD, USA aut NATIONALLICENCE 700 1- Oyler George Department of Chemical & Biomolecular Engineering, Johns Hopkins University, 3400 North Charles Street, 21218-2686, Baltimore, MD, USA aut NATIONALLICENCE 700 1- Betenbaugh Michael Department of Chemical & Biomolecular Engineering, Johns Hopkins University, 3400 North Charles Street, 21218-2686, Baltimore, MD, USA aut NATIONALLICENCE 700 1- Bouwer Edward Department of Geography & Environmental Engineering, Johns Hopkins University, 3400 North Charles Street, 21218-2686, Baltimore, MD, USA aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/14(2015-07-01), 6139-6154 0175-7598 99:14<6139 2015 99 253