Untargeted metabolomics analysis revealed changes in the composition of glycerolipids and phospholipids in Bacillus subtilis under 1-butanol stress
| LEADER | caa a22 4500 | ||
|---|---|---|---|
| 001 | 605503370 | ||
| 003 | CHVBK | ||
| 005 | 20210128100610.0 | ||
| 007 | cr unu---uuuuu | ||
| 008 | 210128e20150701xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.1007/s00253-015-6692-0 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6692-0 | ||
| 245 | 0 | 0 | |a Untargeted metabolomics analysis revealed changes in the composition of glycerolipids and phospholipids in Bacillus subtilis under 1-butanol stress |h [Elektronische Daten] |c [Nawaporn Vinayavekhin, Gumpanat Mahipant, Alisa Vangnai, Polkit Sangvanich] |
| 520 | 3 | |a 1-Butanol has been utilized widely in industry and can be produced or transformed by microbes. However, current knowledge about the mechanisms of 1-butanol tolerance in bacteria remains quite limited. Here, we applied untargeted metabolomics to study Bacillus subtilis cells under 1-butanol stress and identified 55 and 37 ions with significantly increased and decreased levels, respectively. Using accurate mass determination, tandem mass spectra, and synthetic standards, 86% of these ions were characterized. The levels of phosphatidylethanolamine, diglucosyldiacylglycerol, and phosphatidylserine were found to be upregulated upon 1-butanol treatment, whereas those of diacylglycerol and lysyl phosphatidylglycerol were downregulated. Most lipids contained 15:0/15:0, 16:0/15:0, and 17:0/15:0 acyl chains, and all were mapped to membrane lipid biosynthetic pathways. Subsequent two-stage quantitative real-time reverse transcriptase PCR analyses of genes in the two principal membrane lipid biosynthesis pathways revealed elevated levels of ywiE transcripts in the presence of 1-butanol and reduced expression levels of cdsA, pgsA, mprF, clsA, and yfnI transcripts. Thus, the gene transcript levels showed agreement with the metabolomics data. Lastly, the cell morphology was investigated by scanning electron microscopy, which indicated that cells became almost twofold longer after 1.4% (v/v) 1-butanol stress for 12h. Overall, the studies uncovered changes in the composition of glycerolipids and phospholipids in B. subtilis under 1-butanol stress, emphasizing the power of untargeted metabolomics in the discovery of new biological insights. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Metabolomics |2 nationallicence | |
| 690 | 7 | |a Bacillus subtilis |2 nationallicence | |
| 690 | 7 | |a 1-Butanol tolerance |2 nationallicence | |
| 690 | 7 | |a Glycerolipids |2 nationallicence | |
| 690 | 7 | |a Phospholipids |2 nationallicence | |
| 690 | 7 | |a Membrane lipids |2 nationallicence | |
| 700 | 1 | |a Vinayavekhin |D Nawaporn |u Department of Chemistry, Faculty of Science, Chulalongkorn University, 10330, Bangkok, Thailand |4 aut | |
| 700 | 1 | |a Mahipant |D Gumpanat |u Biological Sciences Program, Faculty of Science, Chulalongkorn University, 10330, Bangkok, Thailand |4 aut | |
| 700 | 1 | |a Vangnai |D Alisa |u Department of Biochemistry, Faculty of Science, Chulalongkorn University, 10330, Bangkok, Thailand |4 aut | |
| 700 | 1 | |a Sangvanich |D Polkit |u Department of Chemistry, Faculty of Science, Chulalongkorn University, 10330, Bangkok, Thailand |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/14(2015-07-01), 5971-5983 |x 0175-7598 |q 99:14<5971 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6692-0 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6692-0 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Vinayavekhin |D Nawaporn |u Department of Chemistry, Faculty of Science, Chulalongkorn University, 10330, Bangkok, Thailand |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Mahipant |D Gumpanat |u Biological Sciences Program, Faculty of Science, Chulalongkorn University, 10330, Bangkok, Thailand |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Vangnai |D Alisa |u Department of Biochemistry, Faculty of Science, Chulalongkorn University, 10330, Bangkok, Thailand |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Sangvanich |D Polkit |u Department of Chemistry, Faculty of Science, Chulalongkorn University, 10330, Bangkok, Thailand |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/14(2015-07-01), 5971-5983 |x 0175-7598 |q 99:14<5971 |1 2015 |2 99 |o 253 | ||