caa a22 4500 605503389 CHVBK 20210128100610.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00253-015-6489-1 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6489-1 Improved productivity of poly (3-hydroxybutyrate) (PHB) in thermophilic Chelatococcus daeguensis TAD1 using glycerol as the growth substrate in a fed-batch culture [Elektronische Daten] [Bin Cui, Shaobin Huang, Fuqian Xu, Ruijian Zhang, Yongqing Zhang] A particularly successful polyhydroxyalkanoate (PHA) in industrial applications is poly (3-hydroxybutyrate) (PHB). However, one of the major obstacles for wider application of PHB is the cost of its production and purification. Therefore, it is desirable to discover a method for producing PHB in large quantities at a competitive price. Glycerol is a cheap and widely used carbon source that can be applied in PHB production process. There are numerous advantages to operating fermentation at elevated temperatures; only several thermophilic bacteria are able to accumulate PHB when glycerol is the growth substrate. Here, we report on the possibility of increasing PHB production at low cost using thermophilic Chelatococcus daeguensis TAD1 when glycerol is the growth substrate in a fed-batch culture. We found that (1) excess glycerol inhibited PHB accumulation and (2) organic nitrogen sources, such as tryptone and yeast extract, promoted the growth of C. daeguensis TAD1. In the batch fermentation experiments, we found that using glycerol at low concentrations as the sole carbon source, along with the addition of mixed nitrate (NH4Cl, tryptone, and yeast extract), stimulated PHB accumulation in C. daeguensis TAD1. The results showed that the PHB productivity decreased in the following order: two-stage fed-batch fermentation > fed-batch fermentation > batch fermentation. In optimized culture conditions, a PHB amount of 17.4gl−1 was obtained using a two-stage feeding regimen, leading to a productivity rate of 0.434gl−1h−1, which is the highest productivity rate reported for PHB to date. This high PHB biosynthetic productivity could decrease the total production cost, allowing for further development of industrial applications of PHB. Springer-Verlag Berlin Heidelberg, 2015 PHB accumulation nationallicence Poly(3-hydroxybutyrate) nationallicence Chelatococcus daeguensis TAD1 nationallicence Thermophilic nationallicence Glycerol nationallicence Batch fermentation nationallicence Cui Bin Guangdong Provincial Key Laboratory of Atmospheric Environment and Pollution Control, College of Environment and Energy, South China University of Technology, 510006, Guangzhou, People's Republic of China aut Huang Shaobin Guangdong Provincial Key Laboratory of Atmospheric Environment and Pollution Control, College of Environment and Energy, South China University of Technology, 510006, Guangzhou, People's Republic of China aut Xu Fuqian Guangdong Provincial Key Laboratory of Atmospheric Environment and Pollution Control, College of Environment and Energy, South China University of Technology, 510006, Guangzhou, People's Republic of China aut Zhang Ruijian Guangdong Provincial Key Laboratory of Atmospheric Environment and Pollution Control, College of Environment and Energy, South China University of Technology, 510006, Guangzhou, People's Republic of China aut Zhang Yongqing Guangdong Provincial Key Laboratory of Atmospheric Environment and Pollution Control, College of Environment and Energy, South China University of Technology, 510006, Guangzhou, People's Republic of China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/14(2015-07-01), 6009-6019 0175-7598 99:14<6009 2015 99 253 https://doi.org/10.1007/s00253-015-6489-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6489-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cui Bin Guangdong Provincial Key Laboratory of Atmospheric Environment and Pollution Control, College of Environment and Energy, South China University of Technology, 510006, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Huang Shaobin Guangdong Provincial Key Laboratory of Atmospheric Environment and Pollution Control, College of Environment and Energy, South China University of Technology, 510006, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Xu Fuqian Guangdong Provincial Key Laboratory of Atmospheric Environment and Pollution Control, College of Environment and Energy, South China University of Technology, 510006, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Ruijian Guangdong Provincial Key Laboratory of Atmospheric Environment and Pollution Control, College of Environment and Energy, South China University of Technology, 510006, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Yongqing Guangdong Provincial Key Laboratory of Atmospheric Environment and Pollution Control, College of Environment and Energy, South China University of Technology, 510006, Guangzhou, People's Republic of China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/14(2015-07-01), 6009-6019 0175-7598 99:14<6009 2015 99 253