caa a22 4500 605503397 CHVBK 20210128100610.0 cr unu---uuuuu 210128e20150701xx s 000 0 eng 10.1007/s00253-015-6462-z doi (NATIONALLICENCE)springer-10.1007/s00253-015-6462-z Effects of bioaugmentation in para -nitrophenol-contaminated soil on the abundance and community structure of ammonia-oxidizing bacteria and archaea [Elektronische Daten] [Xiang-Qun Chi, Kun Liu, Ning-Yi Zhou] Pseudomonas sp. strain WBC-3 mineralizes the priority pollutant para-nitrophenol (PNP) and releases nitrite (NO2 −), which is probably involved in the nitrification. In this study, the rate of PNP removal in soil bioaugmented with strain WBC-3 was more accelerated with more NO2 − accumulation than in uninoculated soils. Strain WBC-3 survived well and remained stable throughout the entire period. Real-time polymerase chain reaction (real-time PCR) indicated a higher abundance of ammonia-oxidizing bacteria (AOB) than ammonia-oxidizing archaea (AOA), suggesting that AOB played a greater role in nitrification in the original sampled soil. Real-time PCR and multivariate analysis based on the denaturing gradient gel electrophoresis showed that PNP contamination did not significantly alter the abundance and community structure of ammonia oxidizers except for inhibiting the AOB abundance. Bioaugmentation of PNP-contaminated soil showed a significant effect on AOB populations and community structure as well as AOA populations. In addition, ammonium (NH4 +) variation was found to be the primary factor affecting the AOB community structure, as determined by the correlation between the community structures of ammonia oxidizers and environmental factors. It is here proposed that the balance between archaeal and bacterial ammonia oxidation could be influenced significantly by the variation in NH4 + levels as caused by bioaugmentation of contaminated soil by a pollutant containing nitrogen. Springer-Verlag Berlin Heidelberg, 2015 Ammonia-oxidizing archaea nationallicence Ammonia-oxidizing bacteria nationallicence Bioaugmentation nationallicence Multivariate analyses nationallicence para -nitrophenol nationallicence Pseudomonas sp. strain WBC-3 nationallicence Chi Xiang-Qun Key Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, 430071, Wuhan, China aut Liu Kun State Key Laboratory of Microbial Metabolism and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 200240, Shanghai, China aut Zhou Ning-Yi Key Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, 430071, Wuhan, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/14(2015-07-01), 6069-6082 0175-7598 99:14<6069 2015 99 253 https://doi.org/10.1007/s00253-015-6462-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6462-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chi Xiang-Qun Key Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, 430071, Wuhan, China aut NATIONALLICENCE 700 1- Liu Kun State Key Laboratory of Microbial Metabolism and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 200240, Shanghai, China aut NATIONALLICENCE 700 1- Zhou Ning-Yi Key Laboratory of Agricultural and Environmental Microbiology, Wuhan Institute of Virology, Chinese Academy of Sciences, 430071, Wuhan, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/14(2015-07-01), 6069-6082 0175-7598 99:14<6069 2015 99 253