Dynamics of biomass composition and growth during recovery of nitrogen-starved Chromochloris zofingiensis
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| 024 | 7 | 0 | |a 10.1007/s00253-014-6181-x |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-014-6181-x | ||
| 245 | 0 | 0 | |a Dynamics of biomass composition and growth during recovery of nitrogen-starved Chromochloris zofingiensis |h [Elektronische Daten] |c [Kim Mulders, Packo Lamers, René Wijffels, Dirk Martens] |
| 520 | 3 | |a The effect of nitrogen replenishment on the kinetics of secondary carotenoids, triacylglycerol (TAG) and primary cell components was studied in nitrogen-starved Chromochloris zofingiensis (Chlorophyta), an oleaginous and carotenogenic microalga. Nitrogen resupplied after a period of starvation was initially consumed at a more than four times higher rate than in an equivalent nitrogen-replete culture. Simultaneously, chlorophylls, primary carotenoids, polar (membrane) lipids and proteins were rapidly produced. After 2days, the contents of these primary metabolites, as well as the nitrogen consumption rate and the overall biomass production rate, had returned to values equivalent to those of cells grown under nitrogen-replete conditions, indicating that culture recovery required 2days. Nitrogen resupply was immediately followed by rapid degradation of TAG and starch, suggesting that these metabolites served as carbon and energy source for the recovery process. Also, the secondary carotenoids canthaxanthin and ketolutein were rapidly degraded upon nitrogen resupply, whereas degradation of astaxanthin, the main secondary carotenoid, started only when the cells were fully recovered 2days after nitrogen resupply. This is the first time that such culture recovery has been described in detail and, moreover, that astaxanthin was found to be not immediately degraded after nitrogen resupply. The observed rapid recovery of C. zofingiensis and the delay in astaxanthin degradation suggest that a repeated batch cultivation may result in a higher secondary carotenoid productivity than a series of classical single batch cultivations. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2014 | ||
| 690 | 7 | |a Astaxanthin |2 nationallicence | |
| 690 | 7 | |a Carotenoids |2 nationallicence | |
| 690 | 7 | |a Chromochloris zofingiensis |2 nationallicence | |
| 690 | 7 | |a Nitrogen resupply |2 nationallicence | |
| 690 | 7 | |a Triacylglycerol (TAG) |2 nationallicence | |
| 690 | 7 | |a Recovery |2 nationallicence | |
| 700 | 1 | |a Mulders |D Kim |u Wageningen University, Bioprocess Engineering, AlgaePARC, P.O. Box 8129, 6700 EV, Wageningen, The Netherlands |4 aut | |
| 700 | 1 | |a Lamers |D Packo |u Wageningen University, Bioprocess Engineering, AlgaePARC, P.O. Box 8129, 6700 EV, Wageningen, The Netherlands |4 aut | |
| 700 | 1 | |a Wijffels |D René |u Wageningen University, Bioprocess Engineering, AlgaePARC, P.O. Box 8129, 6700 EV, Wageningen, The Netherlands |4 aut | |
| 700 | 1 | |a Martens |D Dirk |u Wageningen University, Bioprocess Engineering, AlgaePARC, P.O. Box 8129, 6700 EV, Wageningen, The Netherlands |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/4(2015-02-01), 1873-1884 |x 0175-7598 |q 99:4<1873 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-014-6181-x |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-014-6181-x |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Mulders |D Kim |u Wageningen University, Bioprocess Engineering, AlgaePARC, P.O. Box 8129, 6700 EV, Wageningen, The Netherlands |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Lamers |D Packo |u Wageningen University, Bioprocess Engineering, AlgaePARC, P.O. Box 8129, 6700 EV, Wageningen, The Netherlands |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Wijffels |D René |u Wageningen University, Bioprocess Engineering, AlgaePARC, P.O. Box 8129, 6700 EV, Wageningen, The Netherlands |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Martens |D Dirk |u Wageningen University, Bioprocess Engineering, AlgaePARC, P.O. Box 8129, 6700 EV, Wageningen, The Netherlands |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/4(2015-02-01), 1873-1884 |x 0175-7598 |q 99:4<1873 |1 2015 |2 99 |o 253 | ||