Fermentative production of 1-propanol from sugars using wild-type and recombinant Shimwellia blattae

Verfasser / Beitragende:
[Nobuyuki Urano, Misaki Fujii, Hiroshi Kaino, Mitsuru Matsubara, Michihiko Kataoka]
Ort, Verlag, Jahr:
2015
Enthalten in:
Applied Microbiology and Biotechnology, 99/4(2015-02-01), 2001-2008
Format:
Artikel (online)
ID: 605503559
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024 7 0 |a 10.1007/s00253-014-6330-2  |2 doi 
035 |a (NATIONALLICENCE)springer-10.1007/s00253-014-6330-2 
245 0 0 |a Fermentative production of 1-propanol from sugars using wild-type and recombinant Shimwellia blattae  |h [Elektronische Daten]  |c [Nobuyuki Urano, Misaki Fujii, Hiroshi Kaino, Mitsuru Matsubara, Michihiko Kataoka] 
520 3 |a Shimwellia blattae is an enteric bacterium and produces endogenous enzymes that convert 1,2-propanediol (1,2-PD) to 1-propanol, which is expected to be used as a fuel substitute and a precursor of polypropylene. Therefore, if S. blattae could be induced to generate its own 1,2-PD from sugars, it might be possible to produce 1-propanol from sugars with this microorganism. Here, two 1,2-PD production pathways were constructed in S. blattae, resulting in two methods for 1-propanol production with the bacterium. One method employed the L-rhamnose utilization pathway, in which L-rhamnose is split into dihydroxyacetone phosphate and 1,2-PD. When wild-type S. blattae was cultured with L-rhamnose, an accumulation of 1,2-PD was observed. The other method for producing 1,2-PD was to introduce an engineered 1,2-PD production pathway from glucose into S. blattae. In both cases, the produced 1,2-PD was then converted to 1-propanol by 1,2-PD converting enzymes, whose production was induced by the addition of glycerol. 
540 |a Springer-Verlag Berlin Heidelberg, 2014 
690 7 |a Shimwellia blattae  |2 nationallicence 
690 7 |a 1-Propanol production  |2 nationallicence 
690 7 |a 1,2-Propanediol production  |2 nationallicence 
690 7 |a Glycerol dehydratase  |2 nationallicence 
700 1 |a Urano  |D Nobuyuki  |u Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuencho, Naka-ku, 599-8531, Sakai, Osaka, Japan  |4 aut 
700 1 |a Fujii  |D Misaki  |u Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuencho, Naka-ku, 599-8531, Sakai, Osaka, Japan  |4 aut 
700 1 |a Kaino  |D Hiroshi  |u Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuencho, Naka-ku, 599-8531, Sakai, Osaka, Japan  |4 aut 
700 1 |a Matsubara  |D Mitsuru  |u Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuencho, Naka-ku, 599-8531, Sakai, Osaka, Japan  |4 aut 
700 1 |a Kataoka  |D Michihiko  |u Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuencho, Naka-ku, 599-8531, Sakai, Osaka, Japan  |4 aut 
773 0 |t Applied Microbiology and Biotechnology  |d Springer Berlin Heidelberg  |g 99/4(2015-02-01), 2001-2008  |x 0175-7598  |q 99:4<2001  |1 2015  |2 99  |o 253 
856 4 0 |u https://doi.org/10.1007/s00253-014-6330-2  |q text/html  |z Onlinezugriff via DOI 
898 |a BK010053  |b XK010053  |c XK010000 
900 7 |a Metadata rights reserved  |b Springer special CC-BY-NC licence  |2 nationallicence 
908 |D 1  |a research-article  |2 jats 
949 |B NATIONALLICENCE  |F NATIONALLICENCE  |b NL-springer 
950 |B NATIONALLICENCE  |P 856  |E 40  |u https://doi.org/10.1007/s00253-014-6330-2  |q text/html  |z Onlinezugriff via DOI 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Urano  |D Nobuyuki  |u Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuencho, Naka-ku, 599-8531, Sakai, Osaka, Japan  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Fujii  |D Misaki  |u Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuencho, Naka-ku, 599-8531, Sakai, Osaka, Japan  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Kaino  |D Hiroshi  |u Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuencho, Naka-ku, 599-8531, Sakai, Osaka, Japan  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Matsubara  |D Mitsuru  |u Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuencho, Naka-ku, 599-8531, Sakai, Osaka, Japan  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Kataoka  |D Michihiko  |u Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuencho, Naka-ku, 599-8531, Sakai, Osaka, Japan  |4 aut 
950 |B NATIONALLICENCE  |P 773  |E 0-  |t Applied Microbiology and Biotechnology  |d Springer Berlin Heidelberg  |g 99/4(2015-02-01), 2001-2008  |x 0175-7598  |q 99:4<2001  |1 2015  |2 99  |o 253