caa a22 4500 605503559 CHVBK 20210128100611.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s00253-014-6330-2 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6330-2 Fermentative production of 1-propanol from sugars using wild-type and recombinant Shimwellia blattae [Elektronische Daten] [Nobuyuki Urano, Misaki Fujii, Hiroshi Kaino, Mitsuru Matsubara, Michihiko Kataoka] Shimwellia blattae is an enteric bacterium and produces endogenous enzymes that convert 1,2-propanediol (1,2-PD) to 1-propanol, which is expected to be used as a fuel substitute and a precursor of polypropylene. Therefore, if S. blattae could be induced to generate its own 1,2-PD from sugars, it might be possible to produce 1-propanol from sugars with this microorganism. Here, two 1,2-PD production pathways were constructed in S. blattae, resulting in two methods for 1-propanol production with the bacterium. One method employed the L-rhamnose utilization pathway, in which L-rhamnose is split into dihydroxyacetone phosphate and 1,2-PD. When wild-type S. blattae was cultured with L-rhamnose, an accumulation of 1,2-PD was observed. The other method for producing 1,2-PD was to introduce an engineered 1,2-PD production pathway from glucose into S. blattae. In both cases, the produced 1,2-PD was then converted to 1-propanol by 1,2-PD converting enzymes, whose production was induced by the addition of glycerol. Springer-Verlag Berlin Heidelberg, 2014 Shimwellia blattae nationallicence 1-Propanol production nationallicence 1,2-Propanediol production nationallicence Glycerol dehydratase nationallicence Urano Nobuyuki Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuencho, Naka-ku, 599-8531, Sakai, Osaka, Japan aut Fujii Misaki Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuencho, Naka-ku, 599-8531, Sakai, Osaka, Japan aut Kaino Hiroshi Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuencho, Naka-ku, 599-8531, Sakai, Osaka, Japan aut Matsubara Mitsuru Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuencho, Naka-ku, 599-8531, Sakai, Osaka, Japan aut Kataoka Michihiko Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuencho, Naka-ku, 599-8531, Sakai, Osaka, Japan aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/4(2015-02-01), 2001-2008 0175-7598 99:4<2001 2015 99 253 https://doi.org/10.1007/s00253-014-6330-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6330-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Urano Nobuyuki Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuencho, Naka-ku, 599-8531, Sakai, Osaka, Japan aut NATIONALLICENCE 700 1- Fujii Misaki Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuencho, Naka-ku, 599-8531, Sakai, Osaka, Japan aut NATIONALLICENCE 700 1- Kaino Hiroshi Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuencho, Naka-ku, 599-8531, Sakai, Osaka, Japan aut NATIONALLICENCE 700 1- Matsubara Mitsuru Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuencho, Naka-ku, 599-8531, Sakai, Osaka, Japan aut NATIONALLICENCE 700 1- Kataoka Michihiko Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-1 Gakuencho, Naka-ku, 599-8531, Sakai, Osaka, Japan aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/4(2015-02-01), 2001-2008 0175-7598 99:4<2001 2015 99 253