caa a22 4500 605503567 CHVBK 20210128100611.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s00253-014-6234-1 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6234-1 A simple recovery process for biodegradable plastics accumulated in cyanobacteria treated with ionic liquids [Elektronische Daten] [Daigo Kobayashi, Kyoko Fujita, Nobuhumi Nakamura, Hiroyuki Ohno] Here, we proposed a simple recovery process for poly(3-hydroxybutyrate) (PHB) accumulated in cyanobacteria by using ionic liquids (ILs), which dissolve cyanobacteria but not PHB. First, we investigated the effects of IL polarity on hydrogen-bonding receipt ability (β value) and hydrogen-bonding donating ability (α value) and evaluated the subsequent dissolution of cyanobacteria. We found that ILs having α values higher than approximately 0.4 and β values of approximately 0.9 were suitable for dissolution of cyanobacteria. In particular, 1-ethyl-3-methylimidazolium methylphosphonate ([C2mim][MeO(H)PO2]) was found to dissolve cyanobacteria components, but not PHB. Thus, we verified that PHB produced in cyanobacteria could be separated and recovered by simple filtering after dissolution of cyanobacteria in [C2mim][MeO(H)PO2]. Using this technique, more than 98% of PHB was obtained on the filter as residues separated from cyanobacteria. Furthermore, [C2mim][MeO(H)PO2] maintained the ability to dissolve cyanobacteria after a simple recycling procedure. Springer-Verlag Berlin Heidelberg, 2014 Ionic liquid nationallicence Cyanobacteria nationallicence Poly(3-hydroxybutyrate) (PHB) nationallicence Polarity nationallicence Recovery process nationallicence Kobayashi Daigo Department of Biotechnology, Tokyo University of Agriculture, 2-24-16 Naka-cho, 184-8588, Koganei, Tokyo, Japan aut Fujita Kyoko Department of Biotechnology, Tokyo University of Agriculture, 2-24-16 Naka-cho, 184-8588, Koganei, Tokyo, Japan aut Nakamura Nobuhumi Department of Biotechnology, Tokyo University of Agriculture, 2-24-16 Naka-cho, 184-8588, Koganei, Tokyo, Japan aut Ohno Hiroyuki Department of Biotechnology, Tokyo University of Agriculture, 2-24-16 Naka-cho, 184-8588, Koganei, Tokyo, Japan aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/4(2015-02-01), 1647-1653 0175-7598 99:4<1647 2015 99 253 https://doi.org/10.1007/s00253-014-6234-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6234-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kobayashi Daigo Department of Biotechnology, Tokyo University of Agriculture, 2-24-16 Naka-cho, 184-8588, Koganei, Tokyo, Japan aut NATIONALLICENCE 700 1- Fujita Kyoko Department of Biotechnology, Tokyo University of Agriculture, 2-24-16 Naka-cho, 184-8588, Koganei, Tokyo, Japan aut NATIONALLICENCE 700 1- Nakamura Nobuhumi Department of Biotechnology, Tokyo University of Agriculture, 2-24-16 Naka-cho, 184-8588, Koganei, Tokyo, Japan aut NATIONALLICENCE 700 1- Ohno Hiroyuki Department of Biotechnology, Tokyo University of Agriculture, 2-24-16 Naka-cho, 184-8588, Koganei, Tokyo, Japan aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/4(2015-02-01), 1647-1653 0175-7598 99:4<1647 2015 99 253