caa a22 4500 605503605 CHVBK 20210128100611.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s00253-014-6108-6 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6108-6 Enhanced degradation of biphenyl from PCB-contaminated sediments: the impact of extracellular organic matter from Micrococcus luteus [Elektronische Daten] [Xiaomei Su, Qian Zhang, Jinxing Hu, Muhammad Hashmi, Linxian Ding, Chaofeng Shen] Recent advances in the bioremediation of polychlorinated biphenyl (PCB)-contaminated environments have focused on the development of approaches to stimulate the activities of indigenous bacterial communities. In this study, extracellular organic matter (EOM) from Micrococcus luteus was used to enhance the biphenyl-degrading capability of potentially functional microorganisms. The obtained results suggest that EOM significantly enhanced the biphenyl (BP)-degradation capability. Under a concentration of 3,500mg/L BP, BP-degradation efficiency reached 60.8% at a dosage of 10% EOM (v/v), whereas the degradation efficiencies of control group (with inactivated EOM addition) and blank group (with lactate minimal medium) were only 21.5 and 6.2%, respectively. Denaturing gradient gel electrophoresis (DGGE) profiles demonstrated that EOM played a key role in shifts in the composition and diversity of bacterial community. The Illumina high-throughput sequencing analysis indicated that the genera of Rhodococcus and Pseudomonas closely related to BP/PCB-degradation were greatly abundant after EOM addition. Together with polymerase chain reaction (PCR)-DGGE analysis, the link between the enhanced BP-degrading capability and the stimulation and resuscitation function of EOM in uncultured bacteria belonging to phylum Actinobacteria was tentatively established. These results suggest that EOM from M. luteus as an additive holds great potential for the efficient and cost-effective bioremediation of PCB-contaminated environment. Springer-Verlag Berlin Heidelberg, 2014 Micrococcus luteus nationallicence Extracellular organic matter nationallicence Resuscitation-promoting factor nationallicence Biphenyl degradation nationallicence Bacterial community nationallicence Su Xiaomei Department of Environmental Engineering, College of Environmental and Resource Sciences, Zhejiang University, Yuhangtang Road 866, 310058, Hangzhou, China aut Zhang Qian Department of Environmental Engineering, College of Environmental and Resource Sciences, Zhejiang University, Yuhangtang Road 866, 310058, Hangzhou, China aut Hu Jinxing Department of Environmental Engineering, College of Environmental and Resource Sciences, Zhejiang University, Yuhangtang Road 866, 310058, Hangzhou, China aut Hashmi Muhammad Department of Environmental Engineering, College of Environmental and Resource Sciences, Zhejiang University, Yuhangtang Road 866, 310058, Hangzhou, China aut Ding Linxian College of Geography and Environmental Science, Zhejiang Normal University, 321004, Jinhua, China aut Shen Chaofeng Department of Environmental Engineering, College of Environmental and Resource Sciences, Zhejiang University, Yuhangtang Road 866, 310058, Hangzhou, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/4(2015-02-01), 1989-2000 0175-7598 99:4<1989 2015 99 253 https://doi.org/10.1007/s00253-014-6108-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6108-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Su Xiaomei Department of Environmental Engineering, College of Environmental and Resource Sciences, Zhejiang University, Yuhangtang Road 866, 310058, Hangzhou, China aut NATIONALLICENCE 700 1- Zhang Qian Department of Environmental Engineering, College of Environmental and Resource Sciences, Zhejiang University, Yuhangtang Road 866, 310058, Hangzhou, China aut NATIONALLICENCE 700 1- Hu Jinxing Department of Environmental Engineering, College of Environmental and Resource Sciences, Zhejiang University, Yuhangtang Road 866, 310058, Hangzhou, China aut NATIONALLICENCE 700 1- Hashmi Muhammad Department of Environmental Engineering, College of Environmental and Resource Sciences, Zhejiang University, Yuhangtang Road 866, 310058, Hangzhou, China aut NATIONALLICENCE 700 1- Ding Linxian College of Geography and Environmental Science, Zhejiang Normal University, 321004, Jinhua, China aut NATIONALLICENCE 700 1- Shen Chaofeng Department of Environmental Engineering, College of Environmental and Resource Sciences, Zhejiang University, Yuhangtang Road 866, 310058, Hangzhou, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/4(2015-02-01), 1989-2000 0175-7598 99:4<1989 2015 99 253