Elevated level of the second messenger c-di-GMP in Comamonas testosteroni enhances biofilm formation and biofilm-based biodegradation of 3-chloroaniline
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| 024 | 7 | 0 | |a 10.1007/s00253-014-6107-7 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-014-6107-7 | ||
| 245 | 0 | 0 | |a Elevated level of the second messenger c-di-GMP in Comamonas testosteroni enhances biofilm formation and biofilm-based biodegradation of 3-chloroaniline |h [Elektronische Daten] |c [Yichao Wu, Yuanzhao Ding, Yehuda Cohen, Bin Cao] |
| 520 | 3 | |a The bis-(3′-5′)-cyclic dimeric guanosine monophosphate (c-di-GMP) is a ubiquitous second messenger that determines bacterial lifestyle between the planktonic and biofilm modes of life. Although the role of c-di-GMP signaling in biofilm development and dispersal has been extensively studied, how c-di-GMP signaling influences environmental bioprocess activities such as biodegradation remains unexplored. To elucidate the impacts of elevating c-di-GMP level on environmental bioprocesses, we constructed a Comamonas testosteroni strain constitutively expressing a c-di-GMP synthase YedQ from Escherichia coli and examined its capability in biofilm formation and biodegradation of 3-chloroaniline (3-CA). The high c-di-GMP strain exhibited an increased binding to Congo red dye, a decreased motility, and an enhanced biofilm formation capability. In planktonic cultures, the strain with an elevated c-di-GMP concentration and the wild type could degrade 3-CA comparably well. However, under batch growth conditions with a high surface to volume ratio, an elevated c-di-GMP concentration in C. testosteroni significantly increased the contribution of biofilms in 3-CA biodegradation. In continuous submerged biofilm reactors, C. testosteroni with an elevated c-di-GMP level exhibited an enhanced 3-CA biodegradation and a decreased cell detachment rate. Taken together, this study provides a novel strategy to enhance biofilm-based biodegradation of toxic xenobiotic compounds through manipulating bacterial c-di-GMP signaling. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2014 | ||
| 690 | 7 | |a Biofilm |2 nationallicence | |
| 690 | 7 | |a Biodegradation |2 nationallicence | |
| 690 | 7 | |a c-di-GMP |2 nationallicence | |
| 690 | 7 | |a Xenobiotic compound |2 nationallicence | |
| 700 | 1 | |a Wu |D Yichao |u School of Civil and Environmental Engineering, Nanyang Technological University, Singapore, Singapore |4 aut | |
| 700 | 1 | |a Ding |D Yuanzhao |u Singapore Centre on Environmental Life Sciences Engineering, Nanyang Technological University, Singapore, Singapore |4 aut | |
| 700 | 1 | |a Cohen |D Yehuda |u Singapore Centre on Environmental Life Sciences Engineering, Nanyang Technological University, Singapore, Singapore |4 aut | |
| 700 | 1 | |a Cao |D Bin |u School of Civil and Environmental Engineering, Nanyang Technological University, Singapore, Singapore |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/4(2015-02-01), 1967-1976 |x 0175-7598 |q 99:4<1967 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-014-6107-7 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-014-6107-7 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Wu |D Yichao |u School of Civil and Environmental Engineering, Nanyang Technological University, Singapore, Singapore |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ding |D Yuanzhao |u Singapore Centre on Environmental Life Sciences Engineering, Nanyang Technological University, Singapore, Singapore |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Cohen |D Yehuda |u Singapore Centre on Environmental Life Sciences Engineering, Nanyang Technological University, Singapore, Singapore |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Cao |D Bin |u School of Civil and Environmental Engineering, Nanyang Technological University, Singapore, Singapore |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/4(2015-02-01), 1967-1976 |x 0175-7598 |q 99:4<1967 |1 2015 |2 99 |o 253 | ||