caa a22 4500 605503613 CHVBK 20210128100611.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s00253-014-6107-7 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6107-7 Elevated level of the second messenger c-di-GMP in Comamonas testosteroni enhances biofilm formation and biofilm-based biodegradation of 3-chloroaniline [Elektronische Daten] [Yichao Wu, Yuanzhao Ding, Yehuda Cohen, Bin Cao] The bis-(3′-5′)-cyclic dimeric guanosine monophosphate (c-di-GMP) is a ubiquitous second messenger that determines bacterial lifestyle between the planktonic and biofilm modes of life. Although the role of c-di-GMP signaling in biofilm development and dispersal has been extensively studied, how c-di-GMP signaling influences environmental bioprocess activities such as biodegradation remains unexplored. To elucidate the impacts of elevating c-di-GMP level on environmental bioprocesses, we constructed a Comamonas testosteroni strain constitutively expressing a c-di-GMP synthase YedQ from Escherichia coli and examined its capability in biofilm formation and biodegradation of 3-chloroaniline (3-CA). The high c-di-GMP strain exhibited an increased binding to Congo red dye, a decreased motility, and an enhanced biofilm formation capability. In planktonic cultures, the strain with an elevated c-di-GMP concentration and the wild type could degrade 3-CA comparably well. However, under batch growth conditions with a high surface to volume ratio, an elevated c-di-GMP concentration in C. testosteroni significantly increased the contribution of biofilms in 3-CA biodegradation. In continuous submerged biofilm reactors, C. testosteroni with an elevated c-di-GMP level exhibited an enhanced 3-CA biodegradation and a decreased cell detachment rate. Taken together, this study provides a novel strategy to enhance biofilm-based biodegradation of toxic xenobiotic compounds through manipulating bacterial c-di-GMP signaling. Springer-Verlag Berlin Heidelberg, 2014 Biofilm nationallicence Biodegradation nationallicence c-di-GMP nationallicence Xenobiotic compound nationallicence Wu Yichao School of Civil and Environmental Engineering, Nanyang Technological University, Singapore, Singapore aut Ding Yuanzhao Singapore Centre on Environmental Life Sciences Engineering, Nanyang Technological University, Singapore, Singapore aut Cohen Yehuda Singapore Centre on Environmental Life Sciences Engineering, Nanyang Technological University, Singapore, Singapore aut Cao Bin School of Civil and Environmental Engineering, Nanyang Technological University, Singapore, Singapore aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/4(2015-02-01), 1967-1976 0175-7598 99:4<1967 2015 99 253 https://doi.org/10.1007/s00253-014-6107-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6107-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wu Yichao School of Civil and Environmental Engineering, Nanyang Technological University, Singapore, Singapore aut NATIONALLICENCE 700 1- Ding Yuanzhao Singapore Centre on Environmental Life Sciences Engineering, Nanyang Technological University, Singapore, Singapore aut NATIONALLICENCE 700 1- Cohen Yehuda Singapore Centre on Environmental Life Sciences Engineering, Nanyang Technological University, Singapore, Singapore aut NATIONALLICENCE 700 1- Cao Bin School of Civil and Environmental Engineering, Nanyang Technological University, Singapore, Singapore aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/4(2015-02-01), 1967-1976 0175-7598 99:4<1967 2015 99 253