Role of pyruvate carboxylase in accumulation of intracellular lipid of the oleaginous yeast Yarrowia lipolytica ACA-DC 50109
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| 024 | 7 | 0 | |a 10.1007/s00253-014-6236-z |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-014-6236-z | ||
| 245 | 0 | 0 | |a Role of pyruvate carboxylase in accumulation of intracellular lipid of the oleaginous yeast Yarrowia lipolytica ACA-DC 50109 |h [Elektronische Daten] |c [Guang-Yuan Wang, Yan Zhang, Zhe Chi, Guang-Lei Liu, Zhi-Peng Wang, Zhen-Ming Chi] |
| 520 | 3 | |a Yarrowia lipolytica ACA-DC 50109 is an oleaginous yeast. In order to know the function of pyruvate carboxylase (PYC) in lipid biosynthesis, the PYC gene cloned from Pichia guilliermondii Pcla22 was overexpressed in the oleaginous yeast. The lipid contents in the wild-type strain ACA-DC 50109 and the transformants P4, P7, and P103 were 30.2% (w/w) 36.5% (w/w), 38.2% (w/w), and 37.9% (w/w). However, the amount of the secreted citric acids by strains ACA-DC 50109, P4, P77, and P103 were 0.5, 10.1, 11.5, and 9.4g/L. In order to reduce the amount of the secreted citric acid, the PYC gene and endogenous ACL1 gene encoding ATP citrate lyase (ACL1) were simultaneously overexpressed in the oleaginous yeast. The lipid contents of the transformants PA19, PA56, PA124 were 44.4% (w/w), 45.3% (w/w), and 43.7% (w/w). At the same time, the amount of the secreted citric acid by the transformants PA19, PA56, and PA124 was reduced to 5.4, 6.2, and 6.3g/L. The PYC and ACL1 activities and their gene transcriptional levels in all the transformants were greatly enhanced compared to those in their wild-type strain ACA-DC 50109. During 10-L fermentation, lipid content in the transformant PA56 was 49.6% (w/w) and the amount of secreted citric acid was 2.9g/L. This meant that PYC and ACL1 can play an important role in accumulation of intracellular lipid of the oleaginous yeast Y. lipolytica ACA-DC 50109. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2014 | ||
| 690 | 7 | |a Lipid |2 nationallicence | |
| 690 | 7 | |a Pyruvate carboxylase (PYC) |2 nationallicence | |
| 690 | 7 | |a ATP citrate lyase (ACL1) |2 nationallicence | |
| 690 | 7 | |a Oleaginous yeast |2 nationallicence | |
| 690 | 7 | |a Y. lipolytica |2 nationallicence | |
| 700 | 1 | |a Wang |D Guang-Yuan |u Unesco Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No. 5, Qingdao, China |4 aut | |
| 700 | 1 | |a Zhang |D Yan |u Unesco Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No. 5, Qingdao, China |4 aut | |
| 700 | 1 | |a Chi |D Zhe |u Unesco Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No. 5, Qingdao, China |4 aut | |
| 700 | 1 | |a Liu |D Guang-Lei |u Unesco Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No. 5, Qingdao, China |4 aut | |
| 700 | 1 | |a Wang |D Zhi-Peng |u Unesco Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No. 5, Qingdao, China |4 aut | |
| 700 | 1 | |a Chi |D Zhen-Ming |u Unesco Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No. 5, Qingdao, China |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/4(2015-02-01), 1637-1645 |x 0175-7598 |q 99:4<1637 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-014-6236-z |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-014-6236-z |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Wang |D Guang-Yuan |u Unesco Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No. 5, Qingdao, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhang |D Yan |u Unesco Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No. 5, Qingdao, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Chi |D Zhe |u Unesco Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No. 5, Qingdao, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Liu |D Guang-Lei |u Unesco Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No. 5, Qingdao, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Wang |D Zhi-Peng |u Unesco Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No. 5, Qingdao, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Chi |D Zhen-Ming |u Unesco Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No. 5, Qingdao, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/4(2015-02-01), 1637-1645 |x 0175-7598 |q 99:4<1637 |1 2015 |2 99 |o 253 | ||