caa a22 4500 605503621 CHVBK 20210128100611.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s00253-014-6236-z doi (NATIONALLICENCE)springer-10.1007/s00253-014-6236-z Role of pyruvate carboxylase in accumulation of intracellular lipid of the oleaginous yeast Yarrowia lipolytica ACA-DC 50109 [Elektronische Daten] [Guang-Yuan Wang, Yan Zhang, Zhe Chi, Guang-Lei Liu, Zhi-Peng Wang, Zhen-Ming Chi] Yarrowia lipolytica ACA-DC 50109 is an oleaginous yeast. In order to know the function of pyruvate carboxylase (PYC) in lipid biosynthesis, the PYC gene cloned from Pichia guilliermondii Pcla22 was overexpressed in the oleaginous yeast. The lipid contents in the wild-type strain ACA-DC 50109 and the transformants P4, P7, and P103 were 30.2% (w/w) 36.5% (w/w), 38.2% (w/w), and 37.9% (w/w). However, the amount of the secreted citric acids by strains ACA-DC 50109, P4, P77, and P103 were 0.5, 10.1, 11.5, and 9.4g/L. In order to reduce the amount of the secreted citric acid, the PYC gene and endogenous ACL1 gene encoding ATP citrate lyase (ACL1) were simultaneously overexpressed in the oleaginous yeast. The lipid contents of the transformants PA19, PA56, PA124 were 44.4% (w/w), 45.3% (w/w), and 43.7% (w/w). At the same time, the amount of the secreted citric acid by the transformants PA19, PA56, and PA124 was reduced to 5.4, 6.2, and 6.3g/L. The PYC and ACL1 activities and their gene transcriptional levels in all the transformants were greatly enhanced compared to those in their wild-type strain ACA-DC 50109. During 10-L fermentation, lipid content in the transformant PA56 was 49.6% (w/w) and the amount of secreted citric acid was 2.9g/L. This meant that PYC and ACL1 can play an important role in accumulation of intracellular lipid of the oleaginous yeast Y. lipolytica ACA-DC 50109. Springer-Verlag Berlin Heidelberg, 2014 Lipid nationallicence Pyruvate carboxylase (PYC) nationallicence ATP citrate lyase (ACL1) nationallicence Oleaginous yeast nationallicence Y. lipolytica nationallicence Wang Guang-Yuan Unesco Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No. 5, Qingdao, China aut Zhang Yan Unesco Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No. 5, Qingdao, China aut Chi Zhe Unesco Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No. 5, Qingdao, China aut Liu Guang-Lei Unesco Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No. 5, Qingdao, China aut Wang Zhi-Peng Unesco Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No. 5, Qingdao, China aut Chi Zhen-Ming Unesco Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No. 5, Qingdao, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/4(2015-02-01), 1637-1645 0175-7598 99:4<1637 2015 99 253 https://doi.org/10.1007/s00253-014-6236-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6236-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Guang-Yuan Unesco Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No. 5, Qingdao, China aut NATIONALLICENCE 700 1- Zhang Yan Unesco Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No. 5, Qingdao, China aut NATIONALLICENCE 700 1- Chi Zhe Unesco Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No. 5, Qingdao, China aut NATIONALLICENCE 700 1- Liu Guang-Lei Unesco Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No. 5, Qingdao, China aut NATIONALLICENCE 700 1- Wang Zhi-Peng Unesco Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No. 5, Qingdao, China aut NATIONALLICENCE 700 1- Chi Zhen-Ming Unesco Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No. 5, Qingdao, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/4(2015-02-01), 1637-1645 0175-7598 99:4<1637 2015 99 253