The influence of oxygen limitation for the production of succinic acid with recombinant strains of Yarrowia lipolytica
| LEADER | caa a22 4500 | ||
|---|---|---|---|
| 001 | 60550363X | ||
| 003 | CHVBK | ||
| 005 | 20210128100611.0 | ||
| 007 | cr unu---uuuuu | ||
| 008 | 210128e20150201xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.1007/s00253-014-6252-z |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-014-6252-z | ||
| 245 | 0 | 4 | |a The influence of oxygen limitation for the production of succinic acid with recombinant strains of Yarrowia lipolytica |h [Elektronische Daten] |c [Benjamin Jost, Martina Holz, Andreas Aurich, Gerold Barth, Thomas Bley, Roland Müller] |
| 520 | 3 | |a The yeast Yarrowia lipolytica is able to produce high amounts of several organic acids such as pyruvic, citric, isocitric, alpha-ketoglutaric, and succinic acid. Here we report on the influence of the reduced activity of succinate dehydrogenase in Y. lipolytica on its ability to produce succinate. The recombinant strains Y. lipolytica H222-AZ1 and H222-AZ2 were created by exchange of the native promoter of the succinate dehydrogenase subunit 2 encoding gene by inducible promoters. During the cultivation of the strain Y. lipolytica H222-AZ1 in shaking flask experiments, it was found that the promoter exchange resulted in an increase in succinic acid (SA) production. Moreover, it was found that the production of SA depends on an additional limitation of oxygen. Fed-batch cultivations in 1-l bioreactors confirmed this fundamental finding. Y. lipolytica H222-AZ1 produced 2gl−1 of SA with oxygen supply and 9.2gl−1 under the limitation of oxygen after 165h. By using a less active promoter in Y. lipolytica H222-AZ2, the production of SA was increased to 25gl−1 with a productivity of 0.152g (l*h)−1 and a selectivity of 67% after 165h. Yields of 2.39g SA per gram biomass and 0.26g SA per gram glycerol were found. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2014 | ||
| 690 | 7 | |a Yarrowia lipolytica |2 nationallicence | |
| 690 | 7 | |a Glycerol |2 nationallicence | |
| 690 | 7 | |a Succinic acid |2 nationallicence | |
| 690 | 7 | |a Succinate dehydrogenase |2 nationallicence | |
| 690 | 7 | |a Oxygen limitation |2 nationallicence | |
| 690 | 7 | |a Dissolved oxygen |2 nationallicence | |
| 700 | 1 | |a Jost |D Benjamin |u Umwelt- und Biotechnologisches Zentrum (UBZ), Helmholtz-Zentrum für Umweltforschung - UFZ, Permoserstraße 15, 04318, Leipzig, Germany |4 aut | |
| 700 | 1 | |a Holz |D Martina |u Institut für Bioorgane Chemie, Heinrich-Heine-Universität Düsseldorf, Forschungszentrum Jülich, 52426, Jülich, Germany |4 aut | |
| 700 | 1 | |a Aurich |D Andreas |u Umwelt- und Biotechnologisches Zentrum (UBZ), Helmholtz-Zentrum für Umweltforschung - UFZ, Permoserstraße 15, 04318, Leipzig, Germany |4 aut | |
| 700 | 1 | |a Barth |D Gerold |u Institut für Mikrobiologie, Technische Universität Dresden, 01062, Dresden, Germany |4 aut | |
| 700 | 1 | |a Bley |D Thomas |u Institut für Lebensmittel- und Bioverfahrenstechnik, Technische Universität Dresden, 01062, Dresden, Germany |4 aut | |
| 700 | 1 | |a Müller |D Roland |u Umwelt- und Biotechnologisches Zentrum (UBZ), Helmholtz-Zentrum für Umweltforschung - UFZ, Permoserstraße 15, 04318, Leipzig, Germany |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/4(2015-02-01), 1675-1686 |x 0175-7598 |q 99:4<1675 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-014-6252-z |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-014-6252-z |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Jost |D Benjamin |u Umwelt- und Biotechnologisches Zentrum (UBZ), Helmholtz-Zentrum für Umweltforschung - UFZ, Permoserstraße 15, 04318, Leipzig, Germany |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Holz |D Martina |u Institut für Bioorgane Chemie, Heinrich-Heine-Universität Düsseldorf, Forschungszentrum Jülich, 52426, Jülich, Germany |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Aurich |D Andreas |u Umwelt- und Biotechnologisches Zentrum (UBZ), Helmholtz-Zentrum für Umweltforschung - UFZ, Permoserstraße 15, 04318, Leipzig, Germany |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Barth |D Gerold |u Institut für Mikrobiologie, Technische Universität Dresden, 01062, Dresden, Germany |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Bley |D Thomas |u Institut für Lebensmittel- und Bioverfahrenstechnik, Technische Universität Dresden, 01062, Dresden, Germany |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Müller |D Roland |u Umwelt- und Biotechnologisches Zentrum (UBZ), Helmholtz-Zentrum für Umweltforschung - UFZ, Permoserstraße 15, 04318, Leipzig, Germany |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/4(2015-02-01), 1675-1686 |x 0175-7598 |q 99:4<1675 |1 2015 |2 99 |o 253 | ||