caa a22 4500 60550363X CHVBK 20210128100611.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s00253-014-6252-z doi (NATIONALLICENCE)springer-10.1007/s00253-014-6252-z The influence of oxygen limitation for the production of succinic acid with recombinant strains of Yarrowia lipolytica [Elektronische Daten] [Benjamin Jost, Martina Holz, Andreas Aurich, Gerold Barth, Thomas Bley, Roland Müller] The yeast Yarrowia lipolytica is able to produce high amounts of several organic acids such as pyruvic, citric, isocitric, alpha-ketoglutaric, and succinic acid. Here we report on the influence of the reduced activity of succinate dehydrogenase in Y. lipolytica on its ability to produce succinate. The recombinant strains Y. lipolytica H222-AZ1 and H222-AZ2 were created by exchange of the native promoter of the succinate dehydrogenase subunit 2 encoding gene by inducible promoters. During the cultivation of the strain Y. lipolytica H222-AZ1 in shaking flask experiments, it was found that the promoter exchange resulted in an increase in succinic acid (SA) production. Moreover, it was found that the production of SA depends on an additional limitation of oxygen. Fed-batch cultivations in 1-l bioreactors confirmed this fundamental finding. Y. lipolytica H222-AZ1 produced 2gl−1 of SA with oxygen supply and 9.2gl−1 under the limitation of oxygen after 165h. By using a less active promoter in Y. lipolytica H222-AZ2, the production of SA was increased to 25gl−1 with a productivity of 0.152g (l*h)−1 and a selectivity of 67% after 165h. Yields of 2.39g SA per gram biomass and 0.26g SA per gram glycerol were found. Springer-Verlag Berlin Heidelberg, 2014 Yarrowia lipolytica nationallicence Glycerol nationallicence Succinic acid nationallicence Succinate dehydrogenase nationallicence Oxygen limitation nationallicence Dissolved oxygen nationallicence Jost Benjamin Umwelt- und Biotechnologisches Zentrum (UBZ), Helmholtz-Zentrum für Umweltforschung - UFZ, Permoserstraße 15, 04318, Leipzig, Germany aut Holz Martina Institut für Bioorgane Chemie, Heinrich-Heine-Universität Düsseldorf, Forschungszentrum Jülich, 52426, Jülich, Germany aut Aurich Andreas Umwelt- und Biotechnologisches Zentrum (UBZ), Helmholtz-Zentrum für Umweltforschung - UFZ, Permoserstraße 15, 04318, Leipzig, Germany aut Barth Gerold Institut für Mikrobiologie, Technische Universität Dresden, 01062, Dresden, Germany aut Bley Thomas Institut für Lebensmittel- und Bioverfahrenstechnik, Technische Universität Dresden, 01062, Dresden, Germany aut Müller Roland Umwelt- und Biotechnologisches Zentrum (UBZ), Helmholtz-Zentrum für Umweltforschung - UFZ, Permoserstraße 15, 04318, Leipzig, Germany aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/4(2015-02-01), 1675-1686 0175-7598 99:4<1675 2015 99 253 https://doi.org/10.1007/s00253-014-6252-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6252-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jost Benjamin Umwelt- und Biotechnologisches Zentrum (UBZ), Helmholtz-Zentrum für Umweltforschung - UFZ, Permoserstraße 15, 04318, Leipzig, Germany aut NATIONALLICENCE 700 1- Holz Martina Institut für Bioorgane Chemie, Heinrich-Heine-Universität Düsseldorf, Forschungszentrum Jülich, 52426, Jülich, Germany aut NATIONALLICENCE 700 1- Aurich Andreas Umwelt- und Biotechnologisches Zentrum (UBZ), Helmholtz-Zentrum für Umweltforschung - UFZ, Permoserstraße 15, 04318, Leipzig, Germany aut NATIONALLICENCE 700 1- Barth Gerold Institut für Mikrobiologie, Technische Universität Dresden, 01062, Dresden, Germany aut NATIONALLICENCE 700 1- Bley Thomas Institut für Lebensmittel- und Bioverfahrenstechnik, Technische Universität Dresden, 01062, Dresden, Germany aut NATIONALLICENCE 700 1- Müller Roland Umwelt- und Biotechnologisches Zentrum (UBZ), Helmholtz-Zentrum für Umweltforschung - UFZ, Permoserstraße 15, 04318, Leipzig, Germany aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/4(2015-02-01), 1675-1686 0175-7598 99:4<1675 2015 99 253