Screening, separating, and completely recovering polyphenol oxidases and other biochemicals from sweet potato wastewater in starch production
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| 024 | 7 | 0 | |a 10.1007/s00253-014-6034-7 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-014-6034-7 | ||
| 245 | 0 | 0 | |a Screening, separating, and completely recovering polyphenol oxidases and other biochemicals from sweet potato wastewater in starch production |h [Elektronische Daten] |c [Shi Cheng, Yi-Feng Zhang, Zhao-Qin Zeng, Jia Lin, Ya-Wen Zhang, He Ni, Hai-Hang Li] |
| 520 | 3 | |a Polyphenol oxidase (PPO) has multiple functions, and the lack of commercially available enzyme sources limits its widespread application in various industries. An accurate PPO assay was developed by HPLC determination of the substrate oxidation. Resources screening indicated that sweet potato (Ipomoea batatas L.) wastewater in starch production has high PPO activity. A procedure was developed for separately recovering PPO, β-amylase, sporamins, and small molecular nutrients (SMNs) from sweet potato wastewater. The wastewater was adjusted to pH 3.5 to precipitate PPO, and then adjusted to 50% acetone to precipitate β-amylase and further to 80% acetone to precipitate sporamins. The SMNs were obtained after acetone recovery. Purified powders of 4.3 × 105units of PPO, 4.0 × 106units of β-amylase, 8.70g sporamins, and 20.2g SMNs were obtained from the wastewater of 1kg sweet potato. More than 50 million tons of sweet potato is used for starch production annually around the world. Through this simple procedure, huge amount of biochemical resources can be recovered from the wastewater, which greatly increases the economic value of the crop and saves the environment. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2014 | ||
| 690 | 7 | |a Polyphenol oxidase |2 nationallicence | |
| 690 | 7 | |a β-Amylase |2 nationallicence | |
| 690 | 7 | |a Sporamins |2 nationallicence | |
| 690 | 7 | |a Screening |2 nationallicence | |
| 690 | 7 | |a Sweet potato |2 nationallicence | |
| 690 | 7 | |a Isolation |2 nationallicence | |
| 690 | 7 | |a Wastewater |2 nationallicence | |
| 700 | 1 | |a Cheng |D Shi |u Guangdong Provincial Key Lab of Biotechnology for Plant Development, College of Life Sciences, and Research and Development Center for Rare Animals, South China Normal University, 510631, Guangzhou, China |4 aut | |
| 700 | 1 | |a Zhang |D Yi-Feng |u Guangdong Provincial Key Lab of Biotechnology for Plant Development, College of Life Sciences, and Research and Development Center for Rare Animals, South China Normal University, 510631, Guangzhou, China |4 aut | |
| 700 | 1 | |a Zeng |D Zhao-Qin |u Guangdong Provincial Key Lab of Biotechnology for Plant Development, College of Life Sciences, and Research and Development Center for Rare Animals, South China Normal University, 510631, Guangzhou, China |4 aut | |
| 700 | 1 | |a Lin |D Jia |u Guangdong Provincial Key Lab of Biotechnology for Plant Development, College of Life Sciences, and Research and Development Center for Rare Animals, South China Normal University, 510631, Guangzhou, China |4 aut | |
| 700 | 1 | |a Zhang |D Ya-Wen |u Guangdong Provincial Key Lab of Biotechnology for Plant Development, College of Life Sciences, and Research and Development Center for Rare Animals, South China Normal University, 510631, Guangzhou, China |4 aut | |
| 700 | 1 | |a Ni |D He |u Guangdong Provincial Key Lab of Biotechnology for Plant Development, College of Life Sciences, and Research and Development Center for Rare Animals, South China Normal University, 510631, Guangzhou, China |4 aut | |
| 700 | 1 | |a Li |D Hai-Hang |u Guangdong Provincial Key Lab of Biotechnology for Plant Development, College of Life Sciences, and Research and Development Center for Rare Animals, South China Normal University, 510631, Guangzhou, China |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/4(2015-02-01), 1745-1753 |x 0175-7598 |q 99:4<1745 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-014-6034-7 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-014-6034-7 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Cheng |D Shi |u Guangdong Provincial Key Lab of Biotechnology for Plant Development, College of Life Sciences, and Research and Development Center for Rare Animals, South China Normal University, 510631, Guangzhou, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhang |D Yi-Feng |u Guangdong Provincial Key Lab of Biotechnology for Plant Development, College of Life Sciences, and Research and Development Center for Rare Animals, South China Normal University, 510631, Guangzhou, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zeng |D Zhao-Qin |u Guangdong Provincial Key Lab of Biotechnology for Plant Development, College of Life Sciences, and Research and Development Center for Rare Animals, South China Normal University, 510631, Guangzhou, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Lin |D Jia |u Guangdong Provincial Key Lab of Biotechnology for Plant Development, College of Life Sciences, and Research and Development Center for Rare Animals, South China Normal University, 510631, Guangzhou, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhang |D Ya-Wen |u Guangdong Provincial Key Lab of Biotechnology for Plant Development, College of Life Sciences, and Research and Development Center for Rare Animals, South China Normal University, 510631, Guangzhou, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ni |D He |u Guangdong Provincial Key Lab of Biotechnology for Plant Development, College of Life Sciences, and Research and Development Center for Rare Animals, South China Normal University, 510631, Guangzhou, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Li |D Hai-Hang |u Guangdong Provincial Key Lab of Biotechnology for Plant Development, College of Life Sciences, and Research and Development Center for Rare Animals, South China Normal University, 510631, Guangzhou, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/4(2015-02-01), 1745-1753 |x 0175-7598 |q 99:4<1745 |1 2015 |2 99 |o 253 | ||