caa a22 4500 605503702 CHVBK 20210128100612.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s00253-014-6034-7 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6034-7 Screening, separating, and completely recovering polyphenol oxidases and other biochemicals from sweet potato wastewater in starch production [Elektronische Daten] [Shi Cheng, Yi-Feng Zhang, Zhao-Qin Zeng, Jia Lin, Ya-Wen Zhang, He Ni, Hai-Hang Li] Polyphenol oxidase (PPO) has multiple functions, and the lack of commercially available enzyme sources limits its widespread application in various industries. An accurate PPO assay was developed by HPLC determination of the substrate oxidation. Resources screening indicated that sweet potato (Ipomoea batatas L.) wastewater in starch production has high PPO activity. A procedure was developed for separately recovering PPO, β-amylase, sporamins, and small molecular nutrients (SMNs) from sweet potato wastewater. The wastewater was adjusted to pH 3.5 to precipitate PPO, and then adjusted to 50% acetone to precipitate β-amylase and further to 80% acetone to precipitate sporamins. The SMNs were obtained after acetone recovery. Purified powders of 4.3 × 105units of PPO, 4.0 × 106units of β-amylase, 8.70g sporamins, and 20.2g SMNs were obtained from the wastewater of 1kg sweet potato. More than 50 million tons of sweet potato is used for starch production annually around the world. Through this simple procedure, huge amount of biochemical resources can be recovered from the wastewater, which greatly increases the economic value of the crop and saves the environment. Springer-Verlag Berlin Heidelberg, 2014 Polyphenol oxidase nationallicence β-Amylase nationallicence Sporamins nationallicence Screening nationallicence Sweet potato nationallicence Isolation nationallicence Wastewater nationallicence Cheng Shi Guangdong Provincial Key Lab of Biotechnology for Plant Development, College of Life Sciences, and Research and Development Center for Rare Animals, South China Normal University, 510631, Guangzhou, China aut Zhang Yi-Feng Guangdong Provincial Key Lab of Biotechnology for Plant Development, College of Life Sciences, and Research and Development Center for Rare Animals, South China Normal University, 510631, Guangzhou, China aut Zeng Zhao-Qin Guangdong Provincial Key Lab of Biotechnology for Plant Development, College of Life Sciences, and Research and Development Center for Rare Animals, South China Normal University, 510631, Guangzhou, China aut Lin Jia Guangdong Provincial Key Lab of Biotechnology for Plant Development, College of Life Sciences, and Research and Development Center for Rare Animals, South China Normal University, 510631, Guangzhou, China aut Zhang Ya-Wen Guangdong Provincial Key Lab of Biotechnology for Plant Development, College of Life Sciences, and Research and Development Center for Rare Animals, South China Normal University, 510631, Guangzhou, China aut Ni He Guangdong Provincial Key Lab of Biotechnology for Plant Development, College of Life Sciences, and Research and Development Center for Rare Animals, South China Normal University, 510631, Guangzhou, China aut Li Hai-Hang Guangdong Provincial Key Lab of Biotechnology for Plant Development, College of Life Sciences, and Research and Development Center for Rare Animals, South China Normal University, 510631, Guangzhou, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/4(2015-02-01), 1745-1753 0175-7598 99:4<1745 2015 99 253 https://doi.org/10.1007/s00253-014-6034-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6034-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cheng Shi Guangdong Provincial Key Lab of Biotechnology for Plant Development, College of Life Sciences, and Research and Development Center for Rare Animals, South China Normal University, 510631, Guangzhou, China aut NATIONALLICENCE 700 1- Zhang Yi-Feng Guangdong Provincial Key Lab of Biotechnology for Plant Development, College of Life Sciences, and Research and Development Center for Rare Animals, South China Normal University, 510631, Guangzhou, China aut NATIONALLICENCE 700 1- Zeng Zhao-Qin Guangdong Provincial Key Lab of Biotechnology for Plant Development, College of Life Sciences, and Research and Development Center for Rare Animals, South China Normal University, 510631, Guangzhou, China aut NATIONALLICENCE 700 1- Lin Jia Guangdong Provincial Key Lab of Biotechnology for Plant Development, College of Life Sciences, and Research and Development Center for Rare Animals, South China Normal University, 510631, Guangzhou, China aut NATIONALLICENCE 700 1- Zhang Ya-Wen Guangdong Provincial Key Lab of Biotechnology for Plant Development, College of Life Sciences, and Research and Development Center for Rare Animals, South China Normal University, 510631, Guangzhou, China aut NATIONALLICENCE 700 1- Ni He Guangdong Provincial Key Lab of Biotechnology for Plant Development, College of Life Sciences, and Research and Development Center for Rare Animals, South China Normal University, 510631, Guangzhou, China aut NATIONALLICENCE 700 1- Li Hai-Hang Guangdong Provincial Key Lab of Biotechnology for Plant Development, College of Life Sciences, and Research and Development Center for Rare Animals, South China Normal University, 510631, Guangzhou, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/4(2015-02-01), 1745-1753 0175-7598 99:4<1745 2015 99 253