Isolation, sequencing, and heterologous expression of the Paecilomyces variotii gene encoding S -hydroxymethylglutathione dehydrogenase ( fldA )
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| 024 | 7 | 0 | |a 10.1007/s00253-014-6203-8 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-014-6203-8 | ||
| 245 | 0 | 0 | |a Isolation, sequencing, and heterologous expression of the Paecilomyces variotii gene encoding S -hydroxymethylglutathione dehydrogenase ( fldA ) |h [Elektronische Daten] |c [Takuji Oka, Yuji Komachi, Kazufumi Ohshima, Yoichi Kawano, Kohsai Fukuda, Kazuhiro Nagahama, Keisuke Ekino, Yoshiyuki Nomura] |
| 520 | 3 | |a The filamentous fungus Paecilomyces variotii NBRC 109023 (teleomorph: Byssochlamys spectabilis NBRC 109023) degrades formaldehyde at concentrations as high as 2.4% (w/v). In many prokaryotes and in all known eukaryotes, formaldehyde degradation is catalyzed by S-hydroxymethylglutathione (S-HMGSH) dehydrogenase. We report here the isolation and characterization of the gene encoding S-HMGSH dehydrogenase activity in P. variotii. The 1.6-kb fldA gene contained 5 introns and 6 exons, and the corresponding cDNA was 1143bp, encoding a 40-kDa protein composed of 380 amino acids. FldA was predicted to have 74.3, 73.7, 68.5, and 67.4% amino acid identity to the S-HMGSH dehydrogenases of Hansenula polymorpha, Candida boidinii, Saccharomyces cerevisiae, and Kluyveromyces lactis, respectively. The predicted protein also showed high amino acid similarity (84∼86%) to the products of putative fldA genes from other filamentous fungi, including Aspergillus sp. and Penicillium sp. Notably, the P. variotii fldA gene was able to functionally complement a Saccharomyces cerevisiae strain (BY4741 ∆sfa1) lacking the gene for S-HMGSH dehydrogenase. The heterologous expression construct rendered BY4741 ∆sfa1 tolerant to exogenous formaldehyde. Although BY4741 (parental wild-type strain) was unable to degrade even low concentrations of formaldehyde, BY4741 ∆sfa1 harboring Paecilomyces fldA was able to degrade 4mM formaldehyde within 30h. The findings from this study confirm the essential role of S-HMGSH dehydrogenase in detoxifying formaldehyde. | |
| 540 | |a The Author(s), 2014 | ||
| 690 | 7 | |a Paecilomyces variotii |2 nationallicence | |
| 690 | 7 | |a Formaldehyde degradation |2 nationallicence | |
| 690 | 7 | |a S -hydroxymethylglutathione dehydrogenase |2 nationallicence | |
| 690 | 7 | |a fldA |2 nationallicence | |
| 690 | 7 | |a Heterologous expression |2 nationallicence | |
| 700 | 1 | |a Oka |D Takuji |u Department of Applied Microbial Technology, Faculty of Biotechnology and Life Science, Sojo University, Ikeda 4-22-1, 860-0082, Kumamoto, Japan |4 aut | |
| 700 | 1 | |a Komachi |D Yuji |u Department of Applied Microbial Technology, Faculty of Biotechnology and Life Science, Sojo University, Ikeda 4-22-1, 860-0082, Kumamoto, Japan |4 aut | |
| 700 | 1 | |a Ohshima |D Kazufumi |u Department of Applied Microbial Technology, Faculty of Biotechnology and Life Science, Sojo University, Ikeda 4-22-1, 860-0082, Kumamoto, Japan |4 aut | |
| 700 | 1 | |a Kawano |D Yoichi |u Department of Applied Microbial Technology, Faculty of Biotechnology and Life Science, Sojo University, Ikeda 4-22-1, 860-0082, Kumamoto, Japan |4 aut | |
| 700 | 1 | |a Fukuda |D Kohsai |u Department of Applied Microbial Technology, Faculty of Biotechnology and Life Science, Sojo University, Ikeda 4-22-1, 860-0082, Kumamoto, Japan |4 aut | |
| 700 | 1 | |a Nagahama |D Kazuhiro |u Department of Applied Microbial Technology, Faculty of Biotechnology and Life Science, Sojo University, Ikeda 4-22-1, 860-0082, Kumamoto, Japan |4 aut | |
| 700 | 1 | |a Ekino |D Keisuke |u Department of Applied Microbial Technology, Faculty of Biotechnology and Life Science, Sojo University, Ikeda 4-22-1, 860-0082, Kumamoto, Japan |4 aut | |
| 700 | 1 | |a Nomura |D Yoshiyuki |u Department of Applied Microbial Technology, Faculty of Biotechnology and Life Science, Sojo University, Ikeda 4-22-1, 860-0082, Kumamoto, Japan |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/4(2015-02-01), 1755-1763 |x 0175-7598 |q 99:4<1755 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-014-6203-8 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-014-6203-8 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Oka |D Takuji |u Department of Applied Microbial Technology, Faculty of Biotechnology and Life Science, Sojo University, Ikeda 4-22-1, 860-0082, Kumamoto, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Komachi |D Yuji |u Department of Applied Microbial Technology, Faculty of Biotechnology and Life Science, Sojo University, Ikeda 4-22-1, 860-0082, Kumamoto, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ohshima |D Kazufumi |u Department of Applied Microbial Technology, Faculty of Biotechnology and Life Science, Sojo University, Ikeda 4-22-1, 860-0082, Kumamoto, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Kawano |D Yoichi |u Department of Applied Microbial Technology, Faculty of Biotechnology and Life Science, Sojo University, Ikeda 4-22-1, 860-0082, Kumamoto, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Fukuda |D Kohsai |u Department of Applied Microbial Technology, Faculty of Biotechnology and Life Science, Sojo University, Ikeda 4-22-1, 860-0082, Kumamoto, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Nagahama |D Kazuhiro |u Department of Applied Microbial Technology, Faculty of Biotechnology and Life Science, Sojo University, Ikeda 4-22-1, 860-0082, Kumamoto, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ekino |D Keisuke |u Department of Applied Microbial Technology, Faculty of Biotechnology and Life Science, Sojo University, Ikeda 4-22-1, 860-0082, Kumamoto, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Nomura |D Yoshiyuki |u Department of Applied Microbial Technology, Faculty of Biotechnology and Life Science, Sojo University, Ikeda 4-22-1, 860-0082, Kumamoto, Japan |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/4(2015-02-01), 1755-1763 |x 0175-7598 |q 99:4<1755 |1 2015 |2 99 |o 253 | ||