Transcriptional analysis of adaptation to high glucose concentrations in Zymomonas mobilis
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| 024 | 7 | 0 | |a 10.1007/s00253-014-6342-y |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-014-6342-y | ||
| 245 | 0 | 0 | |a Transcriptional analysis of adaptation to high glucose concentrations in Zymomonas mobilis |h [Elektronische Daten] |c [Kun Zhang, Huanhuan Shao, Qinghua Cao, Ming-xiong He, Bo Wu, Hong Feng] |
| 520 | 3 | |a The ethanologenic bacterium Zymomonas mobilis is usually tolerant to high concentrations of glucose. The addition of sorbitol decreases the lag phase and increases ethanol yield and productivity of the bacteria in high glucose concentrations. The molecular mechanisms of adaptation to high glucose concentrations and the effect of sorbitol are still unclear. In this study, microarray analysis was used to study the global transcriptional adaptation responses of Z. mobilis to high glucose concentrations. A total of 235 genes were differentially expressed when 220g/L glucose was added with or without 10mM sorbitol. These genes are involved in diverse aspects of cell metabolism and regulation, including membrane transporters, nitrogen metabolism, and plasmid-encoded genes. However, most differentially expressed genes were downregulated when sorbitol was added. Notably, the transcription of almost all genes involved in the Entner-Doudoroff and ethanol production pathways was not significantly affected. In addition, a prophage and a nitrogen-fixation cluster were significantly induced. These results revealed that Z. mobilis cells responded to high glucose concentrations by regulating the transcriptional levels of genes related to membrane channels and transporters, stress response mechanisms, and metabolic pathways. These data provide insight into the intracellular adaptation responses to high glucose concentrations and reveal strategies to engineer efficient ethanol fermentation in Z. mobilis. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Zymomonas mobilis |2 nationallicence | |
| 690 | 7 | |a Transcription |2 nationallicence | |
| 690 | 7 | |a High concentrations of glucose |2 nationallicence | |
| 690 | 7 | |a Sorbitol |2 nationallicence | |
| 690 | 7 | |a Microarray |2 nationallicence | |
| 700 | 1 | |a Zhang |D Kun |u The Key Laboratory for Bio-Resources and Eco-Environment of Ministry of Education, Sichuan Key Laboratory of Molecular Biology and Biotechnology, College of Life Sciences, Sichuan University, 610064, Chengdu, China |4 aut | |
| 700 | 1 | |a Shao |D Huanhuan |u The Key Laboratory for Bio-Resources and Eco-Environment of Ministry of Education, Sichuan Key Laboratory of Molecular Biology and Biotechnology, College of Life Sciences, Sichuan University, 610064, Chengdu, China |4 aut | |
| 700 | 1 | |a Cao |D Qinghua |u The Key Laboratory for Bio-Resources and Eco-Environment of Ministry of Education, Sichuan Key Laboratory of Molecular Biology and Biotechnology, College of Life Sciences, Sichuan University, 610064, Chengdu, China |4 aut | |
| 700 | 1 | |a He |D Ming-xiong |u Biomass Energy Technology Research Centre, Biogas Institute of Ministry of Agriculture, Section 4-13, Renming Nanlu, 610041, Chengdu, China |4 aut | |
| 700 | 1 | |a Wu |D Bo |u Biomass Energy Technology Research Centre, Biogas Institute of Ministry of Agriculture, Section 4-13, Renming Nanlu, 610041, Chengdu, China |4 aut | |
| 700 | 1 | |a Feng |D Hong |u The Key Laboratory for Bio-Resources and Eco-Environment of Ministry of Education, Sichuan Key Laboratory of Molecular Biology and Biotechnology, College of Life Sciences, Sichuan University, 610064, Chengdu, China |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/4(2015-02-01), 2009-2022 |x 0175-7598 |q 99:4<2009 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-014-6342-y |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-014-6342-y |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhang |D Kun |u The Key Laboratory for Bio-Resources and Eco-Environment of Ministry of Education, Sichuan Key Laboratory of Molecular Biology and Biotechnology, College of Life Sciences, Sichuan University, 610064, Chengdu, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Shao |D Huanhuan |u The Key Laboratory for Bio-Resources and Eco-Environment of Ministry of Education, Sichuan Key Laboratory of Molecular Biology and Biotechnology, College of Life Sciences, Sichuan University, 610064, Chengdu, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Cao |D Qinghua |u The Key Laboratory for Bio-Resources and Eco-Environment of Ministry of Education, Sichuan Key Laboratory of Molecular Biology and Biotechnology, College of Life Sciences, Sichuan University, 610064, Chengdu, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a He |D Ming-xiong |u Biomass Energy Technology Research Centre, Biogas Institute of Ministry of Agriculture, Section 4-13, Renming Nanlu, 610041, Chengdu, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Wu |D Bo |u Biomass Energy Technology Research Centre, Biogas Institute of Ministry of Agriculture, Section 4-13, Renming Nanlu, 610041, Chengdu, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Feng |D Hong |u The Key Laboratory for Bio-Resources and Eco-Environment of Ministry of Education, Sichuan Key Laboratory of Molecular Biology and Biotechnology, College of Life Sciences, Sichuan University, 610064, Chengdu, China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/4(2015-02-01), 2009-2022 |x 0175-7598 |q 99:4<2009 |1 2015 |2 99 |o 253 | ||