Sustainable source of omega-3 eicosapentaenoic acid from metabolically engineered Yarrowia lipolytica
from fundamental research to commercial production
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| 024 | 7 | 0 | |a 10.1007/s00253-014-6318-y |2 doi |
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| 245 | 0 | 0 | |a Sustainable source of omega-3 eicosapentaenoic acid from metabolically engineered Yarrowia lipolytica |h [Elektronische Daten] |b from fundamental research to commercial production |c [Dongming Xie, Ethel Jackson, Quinn Zhu] |
| 520 | 3 | |a The omega-3 fatty acids, cis-5, 8, 11, 14, and 17-eicosapentaenoic acid (C20:5; EPA) and cis-4, 7, 10, 13, 16, and 19-docosahexaenoic acid (C22:6; DHA), have wide-ranging benefits in improving heart health, immune function, mental health, and infant cognitive development. Currently, the major source for EPA and DHA is from fish oil, and a minor source of DHA is from microalgae. With the increased demand for EPA and DHA, DuPont has developed a clean and sustainable source of the omega-3 fatty acid EPA through fermentation using metabolically engineered strains of Yarrowia lipolytica. In this mini-review, we will focus on DuPont's technology for EPA production. Specifically, EPA biosynthetic and supporting pathways have been introduced into the oleaginous yeast to synthesize and accumulate EPA under fermentation conditions. This Yarrowia platform can also produce tailored omega-3 (EPA, DHA) and/or omega-6 (ARA, GLA) fatty acid mixtures in the cellular lipid profiles. Fundamental research such as metabolic engineering for strain construction, high-throughput screening for strain selection, fermentation process development, and process scale-up were all needed to achieve the high levels of EPA titer, rate, and yield required for commercial application. Here, we summarize how we have combined the fundamental bioscience and the industrial engineering skills to achieve large-scale production of Yarrowia biomass containing high amounts of EPA, which led to two commercial products, New Harvest™ EPA oil and Verlasso® salmon. | |
| 540 | |a The Author(s), 2015 | ||
| 690 | 7 | |a Yarrowia lipolytica |2 nationallicence | |
| 690 | 7 | |a Omega-3 fatty acid |2 nationallicence | |
| 690 | 7 | |a Metabolic engineering |2 nationallicence | |
| 690 | 7 | |a Fermentation |2 nationallicence | |
| 690 | 7 | |a Commercialization |2 nationallicence | |
| 700 | 1 | |a Xie |D Dongming |u Biotechnology, Central Research and Development, E.I. du Pont de Nemours and Company, Wilmington, DE, USA |4 aut | |
| 700 | 1 | |a Jackson |D Ethel |u Biotechnology, Central Research and Development, E.I. du Pont de Nemours and Company, Wilmington, DE, USA |4 aut | |
| 700 | 1 | |a Zhu |D Quinn |u Biotechnology, Central Research and Development, E.I. du Pont de Nemours and Company, Wilmington, DE, USA |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/4(2015-02-01), 1599-1610 |x 0175-7598 |q 99:4<1599 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-014-6318-y |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a review-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-014-6318-y |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Xie |D Dongming |u Biotechnology, Central Research and Development, E.I. du Pont de Nemours and Company, Wilmington, DE, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Jackson |D Ethel |u Biotechnology, Central Research and Development, E.I. du Pont de Nemours and Company, Wilmington, DE, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhu |D Quinn |u Biotechnology, Central Research and Development, E.I. du Pont de Nemours and Company, Wilmington, DE, USA |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/4(2015-02-01), 1599-1610 |x 0175-7598 |q 99:4<1599 |1 2015 |2 99 |o 253 | ||