caa a22 4500 605503796 CHVBK 20210128100612.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s00253-014-6096-6 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6096-6 Effect of hydraulic retention time (HRT) on the biodegradation of trichloroethylene wastewater and anaerobic bacterial community in the UASB reactor [Elektronische Daten] [Ying Zhang, Xin Wang, Miao Hu, Pengfei Li] This study utilizes the unique merits of an 8-L laboratory upflow anaerobic sludge blanket (UASB) reactor for treating synthetic wastewater containing trichloroethylene (TCE). The reactor was operated at different hydraulic retention times (HRT) of 25, 20, 15, 10, and 5h. TCE removal efficiency decreased from 99 to 85% when the HRT was lowered down from 25 to 5h, as well as chemical oxygen demand (COD) removal efficiency (from 95 to 84.15%). Using Illumina 16S rRNA gene MiSeq sequencing, we investigated the evolution of bacterial communities in the anaerobic sludge under five different conditions of HRT. In total, 106,387 effective sequences of the 16S rRNA gene were generated from 5 samples that widely represented the diversity of microbial community. Sequence analysis consisting of several novel taxonomic levels ranging from phyla to genera revealed the percentages of these bacterial groups in each sample under different HRTs. The differences found among the five samples indicated that HRT had effects on the structures of bacterial communities and the changes of bacterial communities associated with the effect of HRT on the performance of the reactor. Sequence analyses showed that Bacteroidetes and Firmicutes were the dominant phyla. It is notable that the class Dehalococcoidia was found in the samples at HRT of 5, 10, 20, and 25h, respectively, in which there were some dechlorination strains. Moreover, a tremendous rise of TCE removal efficiency from HRT of 5h to HRT of 10h was found. Springer-Verlag Berlin Heidelberg, 2014 Anaerobic granular sludge nationallicence Hydraulic retention time nationallicence Trichloroethylene nationallicence Upflow anaerobic sludge blanket reactor nationallicence High-throughput sequencing nationallicence Zhang Ying School of Resource and Environment, Northeast Agricultural University, 150030, Harbin, China aut Wang Xin School of Resource and Environment, Northeast Agricultural University, 150030, Harbin, China aut Hu Miao School of Resource and Environment, Northeast Agricultural University, 150030, Harbin, China aut Li Pengfei School of Resource and Environment, Northeast Agricultural University, 150030, Harbin, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/4(2015-02-01), 1977-1987 0175-7598 99:4<1977 2015 99 253 https://doi.org/10.1007/s00253-014-6096-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6096-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Ying School of Resource and Environment, Northeast Agricultural University, 150030, Harbin, China aut NATIONALLICENCE 700 1- Wang Xin School of Resource and Environment, Northeast Agricultural University, 150030, Harbin, China aut NATIONALLICENCE 700 1- Hu Miao School of Resource and Environment, Northeast Agricultural University, 150030, Harbin, China aut NATIONALLICENCE 700 1- Li Pengfei School of Resource and Environment, Northeast Agricultural University, 150030, Harbin, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/4(2015-02-01), 1977-1987 0175-7598 99:4<1977 2015 99 253