Transcriptome analysis of rainbow trout in response to non-virion (NV) protein of viral haemorrhagic septicaemia virus (VHSV)
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| 024 | 7 | 0 | |a 10.1007/s00253-014-6366-3 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-014-6366-3 | ||
| 245 | 0 | 0 | |a Transcriptome analysis of rainbow trout in response to non-virion (NV) protein of viral haemorrhagic septicaemia virus (VHSV) |h [Elektronische Daten] |c [Blanca Chinchilla, Paloma Encinas, Amparo Estepa, Julio Coll, Eduardo Gomez-Casado] |
| 520 | 3 | |a The non-virion (NV) protein of viral haemorrhagic septicaemia virus (VHSV), an economically important fish novirhabdovirus, has been implicated in the interference of some host innate mechanisms (i.e. apoptosis) in vitro. This work aimed to characterise the immune-related transcriptome changes in rainbow trout induced by NV protein that have not yet been established in vivo. For that purpose, immune-targeted microarrays were used to analyse the transcriptomes from head kidney and spleen of rainbow trout (Oncorhynchus mykiss) after injection of recombinant NV (rNV). Results showed the extensive downregulation (and in some cases upregulation) of many innate and adaptive immune response genes not related previously to VHSV infection. The newly identified genes belonged to VHSV-induced genes (vigs), tumour necrosis factors, Toll-like receptors, antigen processing and presentation, immune co-stimulatory molecules, interleukins, macrophage chemotaxis, transcription factors, etc. Classification of differentially downregulated genes into rainbow trout immune pathways identified stat1 and jun/atf1 transcription factor genes as the most representative of the multipath gene targets of rNV. Altogether, these results contribute to define the role and effects of NV in trout by orchestrating an immunosuppression of the innate immune responses for favouring viral replication upon VHSV infection. Finally, these transcriptome results open up the possibility to find out new strategies against VHSV and better understand the interrelationships between some immune pathways in trout. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Novirhabdoviruses |2 nationallicence | |
| 690 | 7 | |a VHSV |2 nationallicence | |
| 690 | 7 | |a NV |2 nationallicence | |
| 690 | 7 | |a Transcriptome |2 nationallicence | |
| 690 | 7 | |a Rainbow trout |2 nationallicence | |
| 690 | 7 | |a Targeted-microarrays |2 nationallicence | |
| 690 | 7 | |a stat1 |2 nationallicence | |
| 690 | 7 | |a jun |2 nationallicence | |
| 690 | 7 | |a atf1 |2 nationallicence | |
| 690 | 7 | |a Interferon |2 nationallicence | |
| 690 | 7 | |a mx |2 nationallicence | |
| 690 | 7 | |a Caspases |2 nationallicence | |
| 690 | 7 | |a TLR |2 nationallicence | |
| 690 | 7 | |a VHSV-induced genes (VIG) |2 nationallicence | |
| 690 | 7 | |a Interleukins |2 nationallicence | |
| 690 | 7 | |a Chemokines |2 nationallicence | |
| 690 | 7 | |a Complement |2 nationallicence | |
| 690 | 7 | |a Macrophage |2 nationallicence | |
| 690 | 7 | |a tnf |2 nationallicence | |
| 690 | 7 | |a Antigen presentation machinery |2 nationallicence | |
| 700 | 1 | |a Chinchilla |D Blanca |u Department of Biotechnology, INIA, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, 28040, Madrid, Spain |4 aut | |
| 700 | 1 | |a Encinas |D Paloma |u Department of Biotechnology, INIA, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, 28040, Madrid, Spain |4 aut | |
| 700 | 1 | |a Estepa |D Amparo |u IBMC, Universidad Miguel Hernández, 03202, Elche (Alicante), Spain |4 aut | |
| 700 | 1 | |a Coll |D Julio |u Department of Biotechnology, INIA, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, 28040, Madrid, Spain |4 aut | |
| 700 | 1 | |a Gomez-Casado |D Eduardo |u Department of Biotechnology, INIA, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, 28040, Madrid, Spain |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/4(2015-02-01), 1827-1843 |x 0175-7598 |q 99:4<1827 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-014-6366-3 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-014-6366-3 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Chinchilla |D Blanca |u Department of Biotechnology, INIA, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, 28040, Madrid, Spain |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Encinas |D Paloma |u Department of Biotechnology, INIA, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, 28040, Madrid, Spain |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Estepa |D Amparo |u IBMC, Universidad Miguel Hernández, 03202, Elche (Alicante), Spain |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Coll |D Julio |u Department of Biotechnology, INIA, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, 28040, Madrid, Spain |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Gomez-Casado |D Eduardo |u Department of Biotechnology, INIA, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, 28040, Madrid, Spain |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/4(2015-02-01), 1827-1843 |x 0175-7598 |q 99:4<1827 |1 2015 |2 99 |o 253 | ||