caa a22 4500 605503842 CHVBK 20210128100612.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s00253-014-6366-3 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6366-3 Transcriptome analysis of rainbow trout in response to non-virion (NV) protein of viral haemorrhagic septicaemia virus (VHSV) [Elektronische Daten] [Blanca Chinchilla, Paloma Encinas, Amparo Estepa, Julio Coll, Eduardo Gomez-Casado] The non-virion (NV) protein of viral haemorrhagic septicaemia virus (VHSV), an economically important fish novirhabdovirus, has been implicated in the interference of some host innate mechanisms (i.e. apoptosis) in vitro. This work aimed to characterise the immune-related transcriptome changes in rainbow trout induced by NV protein that have not yet been established in vivo. For that purpose, immune-targeted microarrays were used to analyse the transcriptomes from head kidney and spleen of rainbow trout (Oncorhynchus mykiss) after injection of recombinant NV (rNV). Results showed the extensive downregulation (and in some cases upregulation) of many innate and adaptive immune response genes not related previously to VHSV infection. The newly identified genes belonged to VHSV-induced genes (vigs), tumour necrosis factors, Toll-like receptors, antigen processing and presentation, immune co-stimulatory molecules, interleukins, macrophage chemotaxis, transcription factors, etc. Classification of differentially downregulated genes into rainbow trout immune pathways identified stat1 and jun/atf1 transcription factor genes as the most representative of the multipath gene targets of rNV. Altogether, these results contribute to define the role and effects of NV in trout by orchestrating an immunosuppression of the innate immune responses for favouring viral replication upon VHSV infection. Finally, these transcriptome results open up the possibility to find out new strategies against VHSV and better understand the interrelationships between some immune pathways in trout. Springer-Verlag Berlin Heidelberg, 2015 Novirhabdoviruses nationallicence VHSV nationallicence NV nationallicence Transcriptome nationallicence Rainbow trout nationallicence Targeted-microarrays nationallicence stat1 nationallicence jun nationallicence atf1 nationallicence Interferon nationallicence mx nationallicence Caspases nationallicence TLR nationallicence VHSV-induced genes (VIG) nationallicence Interleukins nationallicence Chemokines nationallicence Complement nationallicence Macrophage nationallicence tnf nationallicence Antigen presentation machinery nationallicence Chinchilla Blanca Department of Biotechnology, INIA, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, 28040, Madrid, Spain aut Encinas Paloma Department of Biotechnology, INIA, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, 28040, Madrid, Spain aut Estepa Amparo IBMC, Universidad Miguel Hernández, 03202, Elche (Alicante), Spain aut Coll Julio Department of Biotechnology, INIA, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, 28040, Madrid, Spain aut Gomez-Casado Eduardo Department of Biotechnology, INIA, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, 28040, Madrid, Spain aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/4(2015-02-01), 1827-1843 0175-7598 99:4<1827 2015 99 253 https://doi.org/10.1007/s00253-014-6366-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6366-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chinchilla Blanca Department of Biotechnology, INIA, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, 28040, Madrid, Spain aut NATIONALLICENCE 700 1- Encinas Paloma Department of Biotechnology, INIA, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, 28040, Madrid, Spain aut NATIONALLICENCE 700 1- Estepa Amparo IBMC, Universidad Miguel Hernández, 03202, Elche (Alicante), Spain aut NATIONALLICENCE 700 1- Coll Julio Department of Biotechnology, INIA, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, 28040, Madrid, Spain aut NATIONALLICENCE 700 1- Gomez-Casado Eduardo Department of Biotechnology, INIA, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, 28040, Madrid, Spain aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/4(2015-02-01), 1827-1843 0175-7598 99:4<1827 2015 99 253