caa a22 4500 605503869 CHVBK 20210128100613.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s00253-014-6261-y doi (NATIONALLICENCE)springer-10.1007/s00253-014-6261-y Clostridium cluster I and their pathogenic members in a full-scale operating biogas plant [Elektronische Daten] [Anja Dohrmann, Meike Walz, Achim Löwen, Christoph Tebbe] A biogas production plant operating with main and secondary digesters (MD, SD) was analysed for the diversity of bacteria from Clostridium cluster I and its pathogenic members. The plant was run in two parallel lines, both receiving silages, and one, in addition, cattle manure (CM). Quantitative PCR of 16S rRNA genes from directly extracted DNA indicated that cluster I represented 0.2 to 5.6% of the total bacterial communities. Its prevalence was particularly low in CM and also in SD compared to MD, indicating its decline during fermentation. In contrast, another highly abundant clostridial group, i.e. the "faecal” cluster XIVa, remained quantitatively unaffected during fermentation. A total of 85.1% of 581,934 rRNA gene sequences gathered by group-specific PCR from the silages, CM and digesters could be assigned to cluster I. All remaining sequences fell into other clostridial groups. The three most dominant operational taxonomic units (OTUs) introduced with CM were from cluster I, and they declined during fermentation. Fermentation with CM significantly increased OTUs of clostridia outside of cluster I but not within. The only OTUs related to pathogens were detected for Clostridium botulinum with 0.18% of all cluster I sequences in maize silage and less than 0.01% in the other substrates and digester materials. These OTUs could be assigned to all four established C. botulinum groups, thus, potentially covering all seven neurotoxins. Mouse lethality tests of samples with suspected presence of C. botulinum, however, indicated no toxigenic potential and, thus, no risk associated with the rare occurrence of these OTUs. Springer-Verlag Berlin Heidelberg, 2014 Biogas plants nationallicence Clostridium botulinum nationallicence Clostridium cluster I nationallicence Clostridium cluster XIVa nationallicence 16S rRNA amplicon analyses nationallicence Pyrosequencing nationallicence Bacterial community analysis nationallicence Cattle manure nationallicence Silages nationallicence Dohrmann Anja Bundesforschungsinstitut für Ländliche Räume, Wald und Fischerei, Thünen Institut für Biodiversität, Bundesallee 50, 38116, Braunschweig, Germany aut Walz Meike Fakultät Ressourcenmanagement, Hochschule für Angewandte Wissenschaften und Kunst (HAWK), Göttingen, Germany aut Löwen Achim Fakultät Ressourcenmanagement, Hochschule für Angewandte Wissenschaften und Kunst (HAWK), Göttingen, Germany aut Tebbe Christoph Bundesforschungsinstitut für Ländliche Räume, Wald und Fischerei, Thünen Institut für Biodiversität, Bundesallee 50, 38116, Braunschweig, Germany aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/8(2015-04-01), 3585-3598 0175-7598 99:8<3585 2015 99 253 https://doi.org/10.1007/s00253-014-6261-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6261-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dohrmann Anja Bundesforschungsinstitut für Ländliche Räume, Wald und Fischerei, Thünen Institut für Biodiversität, Bundesallee 50, 38116, Braunschweig, Germany aut NATIONALLICENCE 700 1- Walz Meike Fakultät Ressourcenmanagement, Hochschule für Angewandte Wissenschaften und Kunst (HAWK), Göttingen, Germany aut NATIONALLICENCE 700 1- Löwen Achim Fakultät Ressourcenmanagement, Hochschule für Angewandte Wissenschaften und Kunst (HAWK), Göttingen, Germany aut NATIONALLICENCE 700 1- Tebbe Christoph Bundesforschungsinstitut für Ländliche Räume, Wald und Fischerei, Thünen Institut für Biodiversität, Bundesallee 50, 38116, Braunschweig, Germany aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/8(2015-04-01), 3585-3598 0175-7598 99:8<3585 2015 99 253