caa a22 4500 605503877 CHVBK 20210128100613.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s00253-015-6519-z doi (NATIONALLICENCE)springer-10.1007/s00253-015-6519-z Comparative genome analysis reveals genetic adaptation to versatile environmental conditions and importance of biofilm lifestyle in Comamonas testosteroni [Elektronische Daten] [Yichao Wu, Krithika Arumugam, Martin Tay, Hari Seshan, Anee Mohanty, Bin Cao] Comamonas testosteroni is an important environmental bacterium capable of degrading a variety of toxic aromatic pollutants and has been demonstrated to be a promising biocatalyst for environmental decontamination. This organism is often found to be among the primary surface colonizers in various natural and engineered ecosystems, suggesting an extraordinary capability of this organism in environmental adaptation and biofilm formation. The goal of this study was to gain genetic insights into the adaption of C. testosteroni to versatile environments and the importance of a biofilm lifestyle. Specifically, a draft genome of C. testosteroni I2 was obtained. The draft genome is 5,778,710bp in length and comprises 110 contigs. The average G+C content was 61.88%. A total of 5365 genes with 5263 protein-coding genes were predicted, whereas 4324 (80.60% of total genes) protein-encoding genes were associated with predicted functions. The catabolic genes responsible for biodegradation of steroid and other aromatic compounds on draft genome were identified. Plasmid pI2 was found to encode a complete pathway for aniline degradation and a partial catabolic pathway for chloroaniline. This organism was found to be equipped with a sophisticated signaling system which helps it find ideal niches and switch between planktonic and biofilm lifestyles. A large number of putative multi-drug-resistant genes coding for abundant outer membrane transporters, chaperones, and heat shock proteins for the protection of cellular function were identified in the genome of strain I2. In addition, the genome of strain I2 was predicted to encode several proteins involved in producing, secreting, and uptaking siderophores under iron-limiting conditions. The genome of strain I2 contains a number of genes responsible for the synthesis and secretion of exopolysaccharides, an extracellular component essential for biofilm formation. Overall, our results reveal the genomic features underlying the adaption of C. testosteroni to versatile environments and highlighting the importance of its biofilm lifestyle. Springer-Verlag Berlin Heidelberg, 2015 Comamonas nationallicence Genome sequence nationallicence Biodegradation nationallicence Biofilm nationallicence Wu Yichao School of Civil and Environmental Engineering, Nanyang Technological University, 639798, Singapore, Singapore aut Arumugam Krithika Singapore Centre on Environmental Life Sciences Engineering, Nanyang Technological University, Singapore, Singapore aut Tay Martin Singapore Centre on Environmental Life Sciences Engineering, Nanyang Technological University, Singapore, Singapore aut Seshan Hari Singapore Centre on Environmental Life Sciences Engineering, Nanyang Technological University, Singapore, Singapore aut Mohanty Anee School of Civil and Environmental Engineering, Nanyang Technological University, 639798, Singapore, Singapore aut Cao Bin School of Civil and Environmental Engineering, Nanyang Technological University, 639798, Singapore, Singapore aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/8(2015-04-01), 3519-3532 0175-7598 99:8<3519 2015 99 253 https://doi.org/10.1007/s00253-015-6519-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6519-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wu Yichao School of Civil and Environmental Engineering, Nanyang Technological University, 639798, Singapore, Singapore aut NATIONALLICENCE 700 1- Arumugam Krithika Singapore Centre on Environmental Life Sciences Engineering, Nanyang Technological University, Singapore, Singapore aut NATIONALLICENCE 700 1- Tay Martin Singapore Centre on Environmental Life Sciences Engineering, Nanyang Technological University, Singapore, Singapore aut NATIONALLICENCE 700 1- Seshan Hari Singapore Centre on Environmental Life Sciences Engineering, Nanyang Technological University, Singapore, Singapore aut NATIONALLICENCE 700 1- Mohanty Anee School of Civil and Environmental Engineering, Nanyang Technological University, 639798, Singapore, Singapore aut NATIONALLICENCE 700 1- Cao Bin School of Civil and Environmental Engineering, Nanyang Technological University, 639798, Singapore, Singapore aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/8(2015-04-01), 3519-3532 0175-7598 99:8<3519 2015 99 253