caa a22 4500 605503893 CHVBK 20210128100613.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s00253-014-6241-2 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6241-2 Impact of the hydrogen partial pressure on lactate degradation in a coculture of Desulfovibrio sp. G11 and Methanobrevibacter arboriphilus DH1 [Elektronische Daten] [H. Junicke, H. Feldman, M. van Loosdrecht, R. Kleerebezem] In this study, the impact of the hydrogen partial pressure on lactate degradation was investigated in a coculture of Desulfovibrio sp. G11 and Methanobrevibacter arboriphilus DH1. To impose a change of the hydrogen partial pressure, formate was added to the reactor. Hydrogen results from the bioconversion of formate besides lactate in the liquid phase. In the presence of a hydrogen-consuming methanogen, this approach allows for a better estimation of low dissolved hydrogen concentrations than under conditions where hydrogen is supplied externally from the gas phase, resulting in a more accurate determination of kinetic parameters. A change of the hydrogen partial pressure from 1,200 to 250ppm resulted in a threefold increase of the biomass-specific lactate consumption rate. The 50% inhibition constant of hydrogen on lactate degradation was determined as 0.692 ± 0.064μM dissolved hydrogen (831 ± 77ppm hydrogen in the gas phase). Moreover, for the first time, the maximum biomass-specific lactate consumption rate of Desulfovibrio sp. G11 (0.083 ± 0.006mol-Lac/mol-XG11/h) and the affinity constant for hydrogen uptake of Methanobrevibacter arboriphilus DH1 (0.601 ± 0.022μM dissolved hydrogen) were determined. Contrary to the widely established view that the biomass-specific growth rate of a methanogenic coculture is determined by the hydrogen-utilizing partner; here, it was found that the hydrogen-producing bacterium determined the biomass-specific growth rate of the coculture grown on lactate and formate. Springer-Verlag Berlin Heidelberg, 2014 Hydrogen inhibition constant nationallicence Syntrophic lactate degradation nationallicence Biomass-specific rates nationallicence Affinity constant for hydrogen uptake nationallicence Interspecies hydrogen transfer nationallicence Junicke H. Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC, Delft, The Netherlands aut Feldman H. Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC, Delft, The Netherlands aut van Loosdrecht M. Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC, Delft, The Netherlands aut Kleerebezem R. Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC, Delft, The Netherlands aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/8(2015-04-01), 3599-3608 0175-7598 99:8<3599 2015 99 253 https://doi.org/10.1007/s00253-014-6241-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6241-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Junicke H. Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC, Delft, The Netherlands aut NATIONALLICENCE 700 1- Feldman H. Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC, Delft, The Netherlands aut NATIONALLICENCE 700 1- van Loosdrecht M. Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC, Delft, The Netherlands aut NATIONALLICENCE 700 1- Kleerebezem R. Department of Biotechnology, Delft University of Technology, Julianalaan 67, 2628 BC, Delft, The Netherlands aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/8(2015-04-01), 3599-3608 0175-7598 99:8<3599 2015 99 253