caa a22 4500 605503915 CHVBK 20210128100613.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s00253-014-6329-8 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6329-8 Continuous nitrous oxide abatement in a novel denitrifying off-gas bioscrubber [Elektronische Daten] [Osvaldo Frutos, Ilan Arvelo, Rebeca Pérez, Guillermo Quijano, Raúl Muñoz] The potential of a bioscrubber composed of a packed bed absorption column coupled to a stirred tank denitrification bioreactor (STR) was assessed for 95days for the continuous abatement of a diluted air emission of N2O at different liquid recycling velocities. N2O removal efficiencies of up to 40 ± 1% were achieved at the highest recirculation velocity (8mh−1) at an empty bed residence time of 3min using a synthetic air emission containing N2O at 104 ± 12 ppmv. N2O was absorbed in the packed bed column and further reduced in the STR at efficiencies >80% using methanol as electron donor. The long-term operation of the bioscrubber suggested that the specialized N2O degrading community established was not able to use N2O as nitrogen source. Additional nitrification assays showed that the activated sludge used as inoculum was not capable of aerobically oxidizing N2O to nitrate or nitrite, regardless of the inorganic carbon concentration tested. Denitrification assays confirmed the ability of non-acclimated activated sludge to readily denitrify N2O at a specific rate of 3.9mg N2O g VSS h-1 using methanol as electron donor. This study constitutes, to the best of our knowledge, the first systematic assessment of the continuous abatement of N2O in air emission. A characterization of the structure of the microbial population in the absorption column by DGGE-sequencing revealed a high microbial diversity and the presence of heterotrophic denitrifying methylotrophs. Springer-Verlag Berlin Heidelberg, 2014 Biofiltration nationallicence Denitrification nationallicence Greenhouse gas nationallicence N2O nationallicence Wastewater treatment plants nationallicence Frutos Osvaldo Department of Chemical Engineering and Environmental Technology, University of Valladolid, Dr. Mergelina, s/n, 47011, Valladolid, Spain aut Arvelo Ilan Department of Chemical Engineering and Environmental Technology, University of Valladolid, Dr. Mergelina, s/n, 47011, Valladolid, Spain aut Pérez Rebeca Department of Chemical Engineering and Environmental Technology, University of Valladolid, Dr. Mergelina, s/n, 47011, Valladolid, Spain aut Quijano Guillermo Department of Chemical Engineering and Environmental Technology, University of Valladolid, Dr. Mergelina, s/n, 47011, Valladolid, Spain aut Muñoz Raúl Department of Chemical Engineering and Environmental Technology, University of Valladolid, Dr. Mergelina, s/n, 47011, Valladolid, Spain aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/8(2015-04-01), 3695-3706 0175-7598 99:8<3695 2015 99 253 https://doi.org/10.1007/s00253-014-6329-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6329-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Frutos Osvaldo Department of Chemical Engineering and Environmental Technology, University of Valladolid, Dr. Mergelina, s/n, 47011, Valladolid, Spain aut NATIONALLICENCE 700 1- Arvelo Ilan Department of Chemical Engineering and Environmental Technology, University of Valladolid, Dr. Mergelina, s/n, 47011, Valladolid, Spain aut NATIONALLICENCE 700 1- Pérez Rebeca Department of Chemical Engineering and Environmental Technology, University of Valladolid, Dr. Mergelina, s/n, 47011, Valladolid, Spain aut NATIONALLICENCE 700 1- Quijano Guillermo Department of Chemical Engineering and Environmental Technology, University of Valladolid, Dr. Mergelina, s/n, 47011, Valladolid, Spain aut NATIONALLICENCE 700 1- Muñoz Raúl Department of Chemical Engineering and Environmental Technology, University of Valladolid, Dr. Mergelina, s/n, 47011, Valladolid, Spain aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/8(2015-04-01), 3695-3706 0175-7598 99:8<3695 2015 99 253