caa a22 4500 605503966 CHVBK 20210128100613.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s00253-014-6275-5 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6275-5 The stability of Pb species during the Pb removal process by growing cells of Phanerochaete chrysosporium [Elektronische Daten] [Guangming Zeng, Ningjie Li, Danlian Huang, Cui Lai, Meihua Zhao, Chao Huang, Zhen Wei, Piao Xu, Chen Zhang, Min Cheng] Extensive studies have been operated on the biosorption of heavy metal using white-rot fungi, whereas information on the stability of the sorbed metal species has never been taken into consideration, which is important for the later disposal of the used biomass. In this study, the growing cells of Phanerochaete chrysosporium were used to remove Pb from the fungal living environment. The bioremoval of Pb proceeded continually until 121h. The bioremoved Pb was found to be stabilized at the first time P. chrysosporium was exposed to Pb ions. The extractable rate of removed Pb decreased constantly and kept at a stable level around 20% after 121h. The results indicated that the growing biomass is efficient for the stabilization of Pb, and the used biomass was suitable to be separated for further disposal at 121h. With environment scanning electron microscopy coupled with energy-dispersive X-ray analysis (ESEM-EDAX) and X-ray powder diffraction (XRD) analysis, the stabilized Pb species were identified to be lead oxalate and lead chloride phosphate. Further, it is found that the stabilization of Pb by growing P. chrysosporium is not strictly limited in the aspect of pH when pH in the environment is in the range of 4-6. Springer-Verlag Berlin Heidelberg, 2014 Heavy metal nationallicence Bioavailability nationallicence Phanerochaete chrysosporium nationallicence Stabilization nationallicence Zeng Guangming College of Environmental Science and Engineering, Hunan University, 410082, Changsha, Hunan, China aut Li Ningjie College of Environmental Science and Engineering, Hunan University, 410082, Changsha, Hunan, China aut Huang Danlian College of Environmental Science and Engineering, Hunan University, 410082, Changsha, Hunan, China aut Lai Cui College of Environmental Science and Engineering, Hunan University, 410082, Changsha, Hunan, China aut Zhao Meihua College of Environmental Science and Engineering, Hunan University, 410082, Changsha, Hunan, China aut Huang Chao College of Environmental Science and Engineering, Hunan University, 410082, Changsha, Hunan, China aut Wei Zhen College of Environmental Science and Engineering, Hunan University, 410082, Changsha, Hunan, China aut Xu Piao College of Environmental Science and Engineering, Hunan University, 410082, Changsha, Hunan, China aut Zhang Chen College of Environmental Science and Engineering, Hunan University, 410082, Changsha, Hunan, China aut Cheng Min College of Environmental Science and Engineering, Hunan University, 410082, Changsha, Hunan, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/8(2015-04-01), 3685-3693 0175-7598 99:8<3685 2015 99 253 https://doi.org/10.1007/s00253-014-6275-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6275-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zeng Guangming College of Environmental Science and Engineering, Hunan University, 410082, Changsha, Hunan, China aut NATIONALLICENCE 700 1- Li Ningjie College of Environmental Science and Engineering, Hunan University, 410082, Changsha, Hunan, China aut NATIONALLICENCE 700 1- Huang Danlian College of Environmental Science and Engineering, Hunan University, 410082, Changsha, Hunan, China aut NATIONALLICENCE 700 1- Lai Cui College of Environmental Science and Engineering, Hunan University, 410082, Changsha, Hunan, China aut NATIONALLICENCE 700 1- Zhao Meihua College of Environmental Science and Engineering, Hunan University, 410082, Changsha, Hunan, China aut NATIONALLICENCE 700 1- Huang Chao College of Environmental Science and Engineering, Hunan University, 410082, Changsha, Hunan, China aut NATIONALLICENCE 700 1- Wei Zhen College of Environmental Science and Engineering, Hunan University, 410082, Changsha, Hunan, China aut NATIONALLICENCE 700 1- Xu Piao College of Environmental Science and Engineering, Hunan University, 410082, Changsha, Hunan, China aut NATIONALLICENCE 700 1- Zhang Chen College of Environmental Science and Engineering, Hunan University, 410082, Changsha, Hunan, China aut NATIONALLICENCE 700 1- Cheng Min College of Environmental Science and Engineering, Hunan University, 410082, Changsha, Hunan, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/8(2015-04-01), 3685-3693 0175-7598 99:8<3685 2015 99 253