The structure and component characteristics of partial nitrification biofilms under autotrophic and heterotrophic conditions

Verfasser / Beitragende:
[Hanli Xu, Cunbao Wang, Zhiwei Liang, Liyi He, Weixiang Wu]
Ort, Verlag, Jahr:
2015
Enthalten in:
Applied Microbiology and Biotechnology, 99/8(2015-04-01), 3673-3683
Format:
Artikel (online)
ID: 605503982
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024 7 0 |a 10.1007/s00253-014-6300-8  |2 doi 
035 |a (NATIONALLICENCE)springer-10.1007/s00253-014-6300-8 
245 0 4 |a The structure and component characteristics of partial nitrification biofilms under autotrophic and heterotrophic conditions  |h [Elektronische Daten]  |c [Hanli Xu, Cunbao Wang, Zhiwei Liang, Liyi He, Weixiang Wu] 
520 3 |a The differences in the structure and component characteristics of partial nitrification biofilms between autotrophic and heterotrophic conditions were investigated in this work. Three-dimensional excitation-emission matrix fluorescence spectroscopy (EEM), fluorescence staining, and confocal laser scanning microscopy (CLSM) were used to determine differences in the architecture and extracellular polymeric substance (EPS) distribution of the autotrophic and heterotrophic biofilms. Partial nitrification was successfully achieved, and the results demonstrated that an appropriate amount of organic carbon (chemical oxygen demand (COD)/N = 2.6) is advantageous for obtaining better partial nitrification. The final ammoniation and nitrosation rates achieved were 97 and 99%, respectively. Proteins (PN) and polysaccharides (PS) were dominant in the tightly bound EPS (TB-EPS) of autotrophic and heterotrophic biofilms, with PN/PS ratios of 0.96 and 0.69, respectively. Proteins, lipids, α-d-glucopyranose polysaccharides, and nucleic acids were mostly present within the layers of biofilms, but they were distributed in the upper-middle portion of the autotrophic biofilm and increased with depth from the upper layer in the heterotrophic biofilms. 
540 |a Springer-Verlag Berlin Heidelberg, 2014 
690 7 |a Extracellular polymeric substances  |2 nationallicence 
690 7 |a Autotrophic biofilms  |2 nationallicence 
690 7 |a Heterotrophic biofilms  |2 nationallicence 
690 7 |a Partial nitrification  |2 nationallicence 
690 7 |a Fluorescence staining  |2 nationallicence 
700 1 |a Xu  |D Hanli  |u Department of Environmental Engineering, Institute of Environmental Science and Technology, Academy of Water Science and Environmental Engineering, Zhejiang University, Zijingang Campus, 866# Yuhangtang Road, 310058, Hangzhou, Zhejiang, People's Republic of China  |4 aut 
700 1 |a Wang  |D Cunbao  |u Department of Environmental Engineering, Institute of Environmental Science and Technology, Academy of Water Science and Environmental Engineering, Zhejiang University, Zijingang Campus, 866# Yuhangtang Road, 310058, Hangzhou, Zhejiang, People's Republic of China  |4 aut 
700 1 |a Liang  |D Zhiwei  |u Department of Environmental Engineering, Institute of Environmental Science and Technology, Academy of Water Science and Environmental Engineering, Zhejiang University, Zijingang Campus, 866# Yuhangtang Road, 310058, Hangzhou, Zhejiang, People's Republic of China  |4 aut 
700 1 |a He  |D Liyi  |u Department of Environmental Engineering, Institute of Environmental Science and Technology, Academy of Water Science and Environmental Engineering, Zhejiang University, Zijingang Campus, 866# Yuhangtang Road, 310058, Hangzhou, Zhejiang, People's Republic of China  |4 aut 
700 1 |a Wu  |D Weixiang  |u Department of Environmental Engineering, Institute of Environmental Science and Technology, Academy of Water Science and Environmental Engineering, Zhejiang University, Zijingang Campus, 866# Yuhangtang Road, 310058, Hangzhou, Zhejiang, People's Republic of China  |4 aut 
773 0 |t Applied Microbiology and Biotechnology  |d Springer Berlin Heidelberg  |g 99/8(2015-04-01), 3673-3683  |x 0175-7598  |q 99:8<3673  |1 2015  |2 99  |o 253 
856 4 0 |u https://doi.org/10.1007/s00253-014-6300-8  |q text/html  |z Onlinezugriff via DOI 
898 |a BK010053  |b XK010053  |c XK010000 
900 7 |a Metadata rights reserved  |b Springer special CC-BY-NC licence  |2 nationallicence 
908 |D 1  |a research-article  |2 jats 
949 |B NATIONALLICENCE  |F NATIONALLICENCE  |b NL-springer 
950 |B NATIONALLICENCE  |P 856  |E 40  |u https://doi.org/10.1007/s00253-014-6300-8  |q text/html  |z Onlinezugriff via DOI 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Xu  |D Hanli  |u Department of Environmental Engineering, Institute of Environmental Science and Technology, Academy of Water Science and Environmental Engineering, Zhejiang University, Zijingang Campus, 866# Yuhangtang Road, 310058, Hangzhou, Zhejiang, People's Republic of China  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Wang  |D Cunbao  |u Department of Environmental Engineering, Institute of Environmental Science and Technology, Academy of Water Science and Environmental Engineering, Zhejiang University, Zijingang Campus, 866# Yuhangtang Road, 310058, Hangzhou, Zhejiang, People's Republic of China  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Liang  |D Zhiwei  |u Department of Environmental Engineering, Institute of Environmental Science and Technology, Academy of Water Science and Environmental Engineering, Zhejiang University, Zijingang Campus, 866# Yuhangtang Road, 310058, Hangzhou, Zhejiang, People's Republic of China  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a He  |D Liyi  |u Department of Environmental Engineering, Institute of Environmental Science and Technology, Academy of Water Science and Environmental Engineering, Zhejiang University, Zijingang Campus, 866# Yuhangtang Road, 310058, Hangzhou, Zhejiang, People's Republic of China  |4 aut 
950 |B NATIONALLICENCE  |P 700  |E 1-  |a Wu  |D Weixiang  |u Department of Environmental Engineering, Institute of Environmental Science and Technology, Academy of Water Science and Environmental Engineering, Zhejiang University, Zijingang Campus, 866# Yuhangtang Road, 310058, Hangzhou, Zhejiang, People's Republic of China  |4 aut 
950 |B NATIONALLICENCE  |P 773  |E 0-  |t Applied Microbiology and Biotechnology  |d Springer Berlin Heidelberg  |g 99/8(2015-04-01), 3673-3683  |x 0175-7598  |q 99:8<3673  |1 2015  |2 99  |o 253