caa a22 4500 605503982 CHVBK 20210128100613.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s00253-014-6300-8 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6300-8 The structure and component characteristics of partial nitrification biofilms under autotrophic and heterotrophic conditions [Elektronische Daten] [Hanli Xu, Cunbao Wang, Zhiwei Liang, Liyi He, Weixiang Wu] The differences in the structure and component characteristics of partial nitrification biofilms between autotrophic and heterotrophic conditions were investigated in this work. Three-dimensional excitation-emission matrix fluorescence spectroscopy (EEM), fluorescence staining, and confocal laser scanning microscopy (CLSM) were used to determine differences in the architecture and extracellular polymeric substance (EPS) distribution of the autotrophic and heterotrophic biofilms. Partial nitrification was successfully achieved, and the results demonstrated that an appropriate amount of organic carbon (chemical oxygen demand (COD)/N = 2.6) is advantageous for obtaining better partial nitrification. The final ammoniation and nitrosation rates achieved were 97 and 99%, respectively. Proteins (PN) and polysaccharides (PS) were dominant in the tightly bound EPS (TB-EPS) of autotrophic and heterotrophic biofilms, with PN/PS ratios of 0.96 and 0.69, respectively. Proteins, lipids, α-d-glucopyranose polysaccharides, and nucleic acids were mostly present within the layers of biofilms, but they were distributed in the upper-middle portion of the autotrophic biofilm and increased with depth from the upper layer in the heterotrophic biofilms. Springer-Verlag Berlin Heidelberg, 2014 Extracellular polymeric substances nationallicence Autotrophic biofilms nationallicence Heterotrophic biofilms nationallicence Partial nitrification nationallicence Fluorescence staining nationallicence Xu Hanli Department of Environmental Engineering, Institute of Environmental Science and Technology, Academy of Water Science and Environmental Engineering, Zhejiang University, Zijingang Campus, 866# Yuhangtang Road, 310058, Hangzhou, Zhejiang, People's Republic of China aut Wang Cunbao Department of Environmental Engineering, Institute of Environmental Science and Technology, Academy of Water Science and Environmental Engineering, Zhejiang University, Zijingang Campus, 866# Yuhangtang Road, 310058, Hangzhou, Zhejiang, People's Republic of China aut Liang Zhiwei Department of Environmental Engineering, Institute of Environmental Science and Technology, Academy of Water Science and Environmental Engineering, Zhejiang University, Zijingang Campus, 866# Yuhangtang Road, 310058, Hangzhou, Zhejiang, People's Republic of China aut He Liyi Department of Environmental Engineering, Institute of Environmental Science and Technology, Academy of Water Science and Environmental Engineering, Zhejiang University, Zijingang Campus, 866# Yuhangtang Road, 310058, Hangzhou, Zhejiang, People's Republic of China aut Wu Weixiang Department of Environmental Engineering, Institute of Environmental Science and Technology, Academy of Water Science and Environmental Engineering, Zhejiang University, Zijingang Campus, 866# Yuhangtang Road, 310058, Hangzhou, Zhejiang, People's Republic of China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/8(2015-04-01), 3673-3683 0175-7598 99:8<3673 2015 99 253 https://doi.org/10.1007/s00253-014-6300-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6300-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xu Hanli Department of Environmental Engineering, Institute of Environmental Science and Technology, Academy of Water Science and Environmental Engineering, Zhejiang University, Zijingang Campus, 866# Yuhangtang Road, 310058, Hangzhou, Zhejiang, People's Republic of China aut NATIONALLICENCE 700 1- Wang Cunbao Department of Environmental Engineering, Institute of Environmental Science and Technology, Academy of Water Science and Environmental Engineering, Zhejiang University, Zijingang Campus, 866# Yuhangtang Road, 310058, Hangzhou, Zhejiang, People's Republic of China aut NATIONALLICENCE 700 1- Liang Zhiwei Department of Environmental Engineering, Institute of Environmental Science and Technology, Academy of Water Science and Environmental Engineering, Zhejiang University, Zijingang Campus, 866# Yuhangtang Road, 310058, Hangzhou, Zhejiang, People's Republic of China aut NATIONALLICENCE 700 1- He Liyi Department of Environmental Engineering, Institute of Environmental Science and Technology, Academy of Water Science and Environmental Engineering, Zhejiang University, Zijingang Campus, 866# Yuhangtang Road, 310058, Hangzhou, Zhejiang, People's Republic of China aut NATIONALLICENCE 700 1- Wu Weixiang Department of Environmental Engineering, Institute of Environmental Science and Technology, Academy of Water Science and Environmental Engineering, Zhejiang University, Zijingang Campus, 866# Yuhangtang Road, 310058, Hangzhou, Zhejiang, People's Republic of China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/8(2015-04-01), 3673-3683 0175-7598 99:8<3673 2015 99 253