caa a22 4500 605503990 CHVBK 20210128100613.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s00253-014-6299-x doi (NATIONALLICENCE)springer-10.1007/s00253-014-6299-x Abundance and diversity of polycyclic aromatic hydrocarbon degradation bacteria in urban roadside soils in Shanghai [Elektronische Daten] [Xiaofei Li, Lijun Hou, Min Liu, Yanling Zheng, Ye Li, Xianbiao Lin] Understanding the impact of polycyclic aromatic hydrocarbons (PAHs) on soil environments is of increasingly important concern. Therefore, the microbial degradation of PAHs in soils has drawn considerable attention, but little is known about the PAH degradation genes in urban soils. In this study, we examined the diversity and abundance of the PAH degradation bacteria and evaluated whether the specific bacteria can reflect PAH contents in the soils from urban roadsides directly receiving traffic emission. The results of phylogenetic analysis indicated that low PAH degradation bacterial diversity occurred in the urban roadside soils, only including Mycobacterium sp., Terrabacter sp., and one novel cluster. The community composition diversity of PAH degradation bacteria did not show a significant difference across the sampling sites. The abundance of PAH degradation genes ranged from 5.70 × 106 to 6.44 × 107genecopiesg−1 dry soil, with an average abundance of 1.43 × 107genecopiesg−1 dry soil, and their spatial variations were related significantly to PAH contents in the soils. The Mycobacterium sp. was the most widely detected and estimated to occupy 65.9-100% of the total PAH degradation bacteria at most of the soil samples, implying that the Mycobacterium sp. might play a primary role in degrading PAHs in the contaminated urban soil environments. Springer-Verlag Berlin Heidelberg, 2014 Polycyclic aromatic hydrocarbons (PAHs) nationallicence Bacterial community nationallicence Degradation nationallicence Urban soil nationallicence Shanghai nationallicence Li Xiaofei College of Geographical Sciences, East China Normal University, 3663 North Zhongshan Road, 200062, Shanghai, China aut Hou Lijun State Key Laboratory of Estuarine and Coastal Research, East China Normal University, 3663 North Zhongshan Road, 200062, Shanghai, China aut Liu Min College of Geographical Sciences, East China Normal University, 3663 North Zhongshan Road, 200062, Shanghai, China aut Zheng Yanling State Key Laboratory of Estuarine and Coastal Research, East China Normal University, 3663 North Zhongshan Road, 200062, Shanghai, China aut Li Ye College of Geographical Sciences, East China Normal University, 3663 North Zhongshan Road, 200062, Shanghai, China aut Lin Xianbiao College of Geographical Sciences, East China Normal University, 3663 North Zhongshan Road, 200062, Shanghai, China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/8(2015-04-01), 3639-3649 0175-7598 99:8<3639 2015 99 253 https://doi.org/10.1007/s00253-014-6299-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6299-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Xiaofei College of Geographical Sciences, East China Normal University, 3663 North Zhongshan Road, 200062, Shanghai, China aut NATIONALLICENCE 700 1- Hou Lijun State Key Laboratory of Estuarine and Coastal Research, East China Normal University, 3663 North Zhongshan Road, 200062, Shanghai, China aut NATIONALLICENCE 700 1- Liu Min College of Geographical Sciences, East China Normal University, 3663 North Zhongshan Road, 200062, Shanghai, China aut NATIONALLICENCE 700 1- Zheng Yanling State Key Laboratory of Estuarine and Coastal Research, East China Normal University, 3663 North Zhongshan Road, 200062, Shanghai, China aut NATIONALLICENCE 700 1- Li Ye College of Geographical Sciences, East China Normal University, 3663 North Zhongshan Road, 200062, Shanghai, China aut NATIONALLICENCE 700 1- Lin Xianbiao College of Geographical Sciences, East China Normal University, 3663 North Zhongshan Road, 200062, Shanghai, China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/8(2015-04-01), 3639-3649 0175-7598 99:8<3639 2015 99 253