caa a22 4500 605504040 CHVBK 20210128100614.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s00253-014-6226-1 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6226-1 Exploring methane-oxidizing communities for the co-metabolic degradation of organic micropollutants [Elektronische Daten] [Jessica Benner, Delfien De Smet, Adrian Ho, Frederiek-Maarten Kerckhof, Lynn Vanhaecke, Kim Heylen, Nico Boon] Methane-oxidizing cultures from five different inocula were enriched to be used for co-metabolic degradation of micropollutants. In a first screening, 18 different compounds were tested for degradation with the cultures as well as with four pure methane-oxidizing bacterial (MOB) strains. The tested compounds included pharmaceuticals, chemical additives, pesticides, and their degradation products. All enriched cultures were successful in the degradation of at least four different pollutants, but the compounds degraded most often were sulfamethoxazole (SMX) and benzotriazole (BTZ). Addition of acetylene, a specific methane monooxygenase (MMO) inhibitor, revealed that SMX and BTZ were mainly degraded co-metabolically by the present MOB. The pure MOB cultures exhibited less degradation potential, while SMX and BTZ were also degraded by three of the four tested pure strains. For MOB, copper (Cu2+) concentration is often an important factor, as several species have the ability to express a soluble MMO (sMMO) if the Cu2+ concentration is low. In literature, this enzyme is often described to have a broader compound range for co-metabolic degradation of pollutants, in particular when it comes to aromatic structures. However, this study indicated that co-metabolic degradation of the aromatic compounds SMX and BTZ was possible at high Cu2+ concentration, most probably catalyzed by pMMO. Springer-Verlag Berlin Heidelberg, 2014 Methanotrophs nationallicence Sulfamethoxazole nationallicence Benzotrialzole nationallicence sMMO nationallicence pMMO nationallicence Copper nationallicence Benner Jessica Laboratory of Microbial Ecology and Technology (LabMET), Department of Biochemical and Microbial Technology, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000, Ghent, Belgium aut De Smet Delfien Laboratory of Microbial Ecology and Technology (LabMET), Department of Biochemical and Microbial Technology, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000, Ghent, Belgium aut Ho Adrian Laboratory of Microbial Ecology and Technology (LabMET), Department of Biochemical and Microbial Technology, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000, Ghent, Belgium aut Kerckhof Frederiek-Maarten Laboratory of Microbial Ecology and Technology (LabMET), Department of Biochemical and Microbial Technology, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000, Ghent, Belgium aut Vanhaecke Lynn Laboratory of Chemical Analysis, Department of Veterinary Public Health and Food Safety, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820, Merelbeke, Belgium aut Heylen Kim Laboratory of Microbiology (LM-UGent), Department of Biochemistry and Microbiology, Faculty of Sciences, Ghent University, K.L. Ledeganckstraat 35, 9000, Ghent, Belgium aut Boon Nico Laboratory of Microbial Ecology and Technology (LabMET), Department of Biochemical and Microbial Technology, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000, Ghent, Belgium aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/8(2015-04-01), 3609-3618 0175-7598 99:8<3609 2015 99 253 https://doi.org/10.1007/s00253-014-6226-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6226-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Benner Jessica Laboratory of Microbial Ecology and Technology (LabMET), Department of Biochemical and Microbial Technology, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000, Ghent, Belgium aut NATIONALLICENCE 700 1- De Smet Delfien Laboratory of Microbial Ecology and Technology (LabMET), Department of Biochemical and Microbial Technology, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000, Ghent, Belgium aut NATIONALLICENCE 700 1- Ho Adrian Laboratory of Microbial Ecology and Technology (LabMET), Department of Biochemical and Microbial Technology, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000, Ghent, Belgium aut NATIONALLICENCE 700 1- Kerckhof Frederiek-Maarten Laboratory of Microbial Ecology and Technology (LabMET), Department of Biochemical and Microbial Technology, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000, Ghent, Belgium aut NATIONALLICENCE 700 1- Vanhaecke Lynn Laboratory of Chemical Analysis, Department of Veterinary Public Health and Food Safety, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820, Merelbeke, Belgium aut NATIONALLICENCE 700 1- Heylen Kim Laboratory of Microbiology (LM-UGent), Department of Biochemistry and Microbiology, Faculty of Sciences, Ghent University, K.L. Ledeganckstraat 35, 9000, Ghent, Belgium aut NATIONALLICENCE 700 1- Boon Nico Laboratory of Microbial Ecology and Technology (LabMET), Department of Biochemical and Microbial Technology, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000, Ghent, Belgium aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/8(2015-04-01), 3609-3618 0175-7598 99:8<3609 2015 99 253