caa a22 4500 605504059 CHVBK 20210128100614.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s00253-015-6373-z doi (NATIONALLICENCE)springer-10.1007/s00253-015-6373-z Copper homeostasis-related genes in three separate transcriptional units regulated by CsoR in Corynebacterium glutamicum [Elektronische Daten] [Haruhiko Teramoto, Hideaki Yukawa, Masayuki Inui] In Corynebacterium glutamicum R, CsoR acts as a transcriptional repressor not only of the cognate copA-csoR operon but also of the copZ1-copB-cgR_0126 operon. It is predicted that copA and copB encode P-type ATPases for copper efflux and copZ1 encodes a metallochaperone. Here, a CsoR-binding motif was found upstream of another copZ-like gene, copZ2, and the in vitro binding of the CsoR protein to its promoter was confirmed. The monocistronic copZ2 transcript was upregulated by excess copper in a CsoR-dependent manner. Among the extended CsoR regulon, deletion of copA, but not of copB, copZ1, or copZ2, resulted in decreased resistance to copper, indicating a major role of the CopA copper exporter in the multilayered systems for copper homeostasis. A redundant role of copZ1 and copZ2 in copper resistance was also indicated by double deletion of these genes. The copper-dependent activation of the copA, copZ1, and copZ2 promoters was confirmed by lacZ reporter assays, consistent with the coordinated derepression of the three transcriptional units. The copZ1 promoter activity showed the highest responsiveness to copper and was also induced by excess zinc and nickel. Furthermore, zinc-inducible expression observed for the CsoR-regulated genes was independent of Zur, recently found to uniquely act as a transcriptional repressor of zinc efflux genes. These results implied complicated cross talk between homeostasis of multiple transition metals. Springer-Verlag Berlin Heidelberg, 2015 Corynebacterium nationallicence Metal homeostasis nationallicence Metal-dependent gene expression nationallicence Transcriptional regulation nationallicence Teramoto Haruhiko Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan aut Yukawa Hideaki Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan aut Inui Masayuki Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/8(2015-04-01), 3505-3517 0175-7598 99:8<3505 2015 99 253 https://doi.org/10.1007/s00253-015-6373-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6373-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Teramoto Haruhiko Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan aut NATIONALLICENCE 700 1- Yukawa Hideaki Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan aut NATIONALLICENCE 700 1- Inui Masayuki Research Institute of Innovative Technology for the Earth, 9-2, Kizugawadai, 619-0292, Kizugawa, Kyoto, Japan aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/8(2015-04-01), 3505-3517 0175-7598 99:8<3505 2015 99 253