Zebrafish gut colonization by mCherry-labelled lactic acid bacteria
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| 008 | 210128e20150401xx s 000 0 eng | ||
| 024 | 7 | 0 | |a 10.1007/s00253-014-6351-x |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-014-6351-x | ||
| 245 | 0 | 0 | |a Zebrafish gut colonization by mCherry-labelled lactic acid bacteria |h [Elektronische Daten] |c [Pasquale Russo, Iñaki Iturria, Maria Mohedano, Graziano Caggianiello, Sandra Rainieri, Daniela Fiocco, Miguel Angel Pardo, Paloma López, Giuseppe Spano] |
| 520 | 3 | |a A critical feature of probiotic microorganisms is their ability to colonize the intestine of the host. Although the microbial potential to adhere to the human gut lumen has been investigated in in vitro models, there is still much to discover about their in vivo behaviour. Zebrafish is a vertebrate model that is being widely used to investigate various biological processes shared with humans. In this work, we report on the use of the zebrafish model to investigate the in vivo colonization ability of previously characterized probiotic lactic acid bacteria. Lactobacillus plantarum Lp90, L. plantarum B2 and Lactobacillus fermentum PBCC11.5 were fluorescently tagged by transfer of the pRCR12 plasmid, which encodes the mCherry protein and which was constructed in this work. The recombinant bacteria were used to infect germ-free zebrafish larvae. After removal of bacteria, the colonization ability of the strains was monitored until 3days post-infection by using a fluorescence stereomicroscope. The results indicated differential adhesion capabilities among the strains. Interestingly, a displacement of bacteria from the medium to the posterior intestinal tract was observed as a function of time that suggested a transient colonization by probiotics. Based on fluorescence observation, L. plantarum strains exhibited a more robust adhesion capability. In conclusion, the use of pRCR12 plasmid for labelling Lactobacillus strains provides a powerful and very efficient tool to monitor the in vivo colonization in zebrafish larvae and to investigate the adhesion ability of probiotic microorganisms. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Zebrafish |2 nationallicence | |
| 690 | 7 | |a Probiotic |2 nationallicence | |
| 690 | 7 | |a In vivo |2 nationallicence | |
| 690 | 7 | |a Lactobacillus plantarum |2 nationallicence | |
| 690 | 7 | |a Lactobacillus fermentum |2 nationallicence | |
| 700 | 1 | |a Russo |D Pasquale |u Promis Biotech srl, via Napoli 25, 71122, Foggia, Italy |4 aut | |
| 700 | 1 | |a Iturria |D Iñaki |u Food Research Unit, Parque Tecnológico de Bizkaia Astondo Bidea, Edif. 609 Derio, Bizkaia, Spain |4 aut | |
| 700 | 1 | |a Mohedano |D Maria |u Department of Molecular Microbiology and Infection Biology, Centro de Investigaciones Biológicas (CIB), Madrid, Spain |4 aut | |
| 700 | 1 | |a Caggianiello |D Graziano |u Department of Agriculture, Food and Environment Sciences, University of Foggia, via Napoli 25, 71122, Foggia, Italy |4 aut | |
| 700 | 1 | |a Rainieri |D Sandra |u Food Research Unit, Parque Tecnológico de Bizkaia Astondo Bidea, Edif. 609 Derio, Bizkaia, Spain |4 aut | |
| 700 | 1 | |a Fiocco |D Daniela |u Department of Clinical and Experimental Medicine, via Pinto, Foggia, Italy |4 aut | |
| 700 | 1 | |a Angel Pardo |D Miguel |u Food Research Unit, Parque Tecnológico de Bizkaia Astondo Bidea, Edif. 609 Derio, Bizkaia, Spain |4 aut | |
| 700 | 1 | |a López |D Paloma |u Department of Molecular Microbiology and Infection Biology, Centro de Investigaciones Biológicas (CIB), Madrid, Spain |4 aut | |
| 700 | 1 | |a Spano |D Giuseppe |u Department of Agriculture, Food and Environment Sciences, University of Foggia, via Napoli 25, 71122, Foggia, Italy |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/8(2015-04-01), 3479-3490 |x 0175-7598 |q 99:8<3479 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-014-6351-x |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-014-6351-x |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Russo |D Pasquale |u Promis Biotech srl, via Napoli 25, 71122, Foggia, Italy |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Iturria |D Iñaki |u Food Research Unit, Parque Tecnológico de Bizkaia Astondo Bidea, Edif. 609 Derio, Bizkaia, Spain |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Mohedano |D Maria |u Department of Molecular Microbiology and Infection Biology, Centro de Investigaciones Biológicas (CIB), Madrid, Spain |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Caggianiello |D Graziano |u Department of Agriculture, Food and Environment Sciences, University of Foggia, via Napoli 25, 71122, Foggia, Italy |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Rainieri |D Sandra |u Food Research Unit, Parque Tecnológico de Bizkaia Astondo Bidea, Edif. 609 Derio, Bizkaia, Spain |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Fiocco |D Daniela |u Department of Clinical and Experimental Medicine, via Pinto, Foggia, Italy |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Angel Pardo |D Miguel |u Food Research Unit, Parque Tecnológico de Bizkaia Astondo Bidea, Edif. 609 Derio, Bizkaia, Spain |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a López |D Paloma |u Department of Molecular Microbiology and Infection Biology, Centro de Investigaciones Biológicas (CIB), Madrid, Spain |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Spano |D Giuseppe |u Department of Agriculture, Food and Environment Sciences, University of Foggia, via Napoli 25, 71122, Foggia, Italy |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/8(2015-04-01), 3479-3490 |x 0175-7598 |q 99:8<3479 |1 2015 |2 99 |o 253 | ||