Enzymatic transformation of vina-ginsenoside R7 to rare notoginsenoside ST-4 using a new recombinant glycoside hydrolase from Herpetosiphon aurantiacus
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| 024 | 7 | 0 | |a 10.1007/s00253-015-6446-z |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6446-z | ||
| 245 | 0 | 0 | |a Enzymatic transformation of vina-ginsenoside R7 to rare notoginsenoside ST-4 using a new recombinant glycoside hydrolase from Herpetosiphon aurantiacus |h [Elektronische Daten] |c [Ru-Feng Wang, Ming-Min Zheng, Yue-De Cao, Hao Li, Chun-Xiu Li, Jian-He Xu, Zheng-Tao Wang] |
| 520 | 3 | |a An eco-friendly and convenient preparation method for notoginsenoside ST-4 has been established by completely transforming vina-ginsenoside R7 using a recombinant glycosidase hydrolyzing enzyme (HaGH03) from Herpetosiphon aurantiacus. This enzyme specifically hydrolyzed the glucose at the C-20 position but not the external xylose or two inner glucoses at position C-3. Protein sequence BLAST revealed that HaGH03, composed of 749 amino acids and presumptively listed as a member of the family 3 glycoside hydrolases, has highest identity (48%) identity with a thermostable β-glucosidase B, which was not known of any functions for ginsenoside transformation. The steady state kinetic parameters for purified HaGH03 measured against p-nitrophenyl β-D-glucopyranoside and vina-ginsenoside R7 were K M = 5.67 ± 0.24μM and 0.59 ± 0.23mM, and k cat = 69.2 ± 0.31/s and 2.15 ± 0.46/min, respectively. HaGH03 converted 2.5mg/mL of vina-ginsenoside R7 to ST-4 with a molar yield of 100% and a space-time yield of 104mg/L/h in optimized conditions. These results underscore that HaGH03 has much potential for the effective preparation of target ginsenosides possessing valuable pharmacological activities. This is the first report identifying an enzyme that has the ability to transform vina-ginsenoside R7 and provides an approach to preparing rare notoginsenoside ST-4. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Biotransformation |2 nationallicence | |
| 690 | 7 | |a Notoginsenoside ST-4 |2 nationallicence | |
| 690 | 7 | |a Vina -ginsenoside R7 |2 nationallicence | |
| 690 | 7 | |a Family 3 glycoside hydrolase |2 nationallicence | |
| 690 | 7 | |a Herpetosiphon aurantiacus |2 nationallicence | |
| 690 | 7 | |a Heterologous expression |2 nationallicence | |
| 700 | 1 | |a Wang |D Ru-Feng |u Department of Pharmacognosy, China Pharmaceutical University, 210038, Nanjing, People's Republic of China |4 aut | |
| 700 | 1 | |a Zheng |D Ming-Min |u State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 200237, Shanghai, People's Republic of China |4 aut | |
| 700 | 1 | |a Cao |D Yue-De |u State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 200237, Shanghai, People's Republic of China |4 aut | |
| 700 | 1 | |a Li |D Hao |u State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 200237, Shanghai, People's Republic of China |4 aut | |
| 700 | 1 | |a Li |D Chun-Xiu |u State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 200237, Shanghai, People's Republic of China |4 aut | |
| 700 | 1 | |a Xu |D Jian-He |u State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 200237, Shanghai, People's Republic of China |4 aut | |
| 700 | 1 | |a Wang |D Zheng-Tao |u Department of Pharmacognosy, China Pharmaceutical University, 210038, Nanjing, People's Republic of China |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/8(2015-04-01), 3433-3442 |x 0175-7598 |q 99:8<3433 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6446-z |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6446-z |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Wang |D Ru-Feng |u Department of Pharmacognosy, China Pharmaceutical University, 210038, Nanjing, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zheng |D Ming-Min |u State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 200237, Shanghai, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Cao |D Yue-De |u State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 200237, Shanghai, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Li |D Hao |u State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 200237, Shanghai, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Li |D Chun-Xiu |u State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 200237, Shanghai, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Xu |D Jian-He |u State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 200237, Shanghai, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Wang |D Zheng-Tao |u Department of Pharmacognosy, China Pharmaceutical University, 210038, Nanjing, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/8(2015-04-01), 3433-3442 |x 0175-7598 |q 99:8<3433 |1 2015 |2 99 |o 253 | ||