Anaerobic humus and Fe(III) reduction and electron transport pathway by a novel humus-reducing bacterium, Thauera humireducens SgZ-1
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| 024 | 7 | 0 | |a 10.1007/s00253-014-6254-x |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-014-6254-x | ||
| 245 | 0 | 0 | |a Anaerobic humus and Fe(III) reduction and electron transport pathway by a novel humus-reducing bacterium, Thauera humireducens SgZ-1 |h [Elektronische Daten] |c [Chen Ma, Zhen Yu, Qin Lu, Li Zhuang, Shun-Gui Zhou] |
| 520 | 3 | |a In this study, an anaerobic batch experiment was conducted to investigate the humus- and Fe(III)-reducing ability of a novel humus-reducing bacterium, Thauera humireducens SgZ-1. Inhibition tests were also performed to explore the electron transport pathways with various electron acceptors. The results indicate that in anaerobic conditions, strain SgZ-1 possesses the ability to reduce a humus analog, humic acids, soluble Fe(III), and Fe(III) oxides. Acetate, propionate, lactate, and pyruvate were suitable electron donors for humus and Fe(III) reduction by strain SgZ-1, while fermentable sugars (glucose and sucrose) were not. UV-visible spectra obtained from intact cells of strain SgZ-1 showed absorption peaks at 420, 522, and 553nm, characteristic of c-type cytochromes (cyt c). Dithionite-reduced cyt c was reoxidized by Fe-EDTA and HFO (hydrous ferric oxide), which suggests that cyt c within intact cells of strain SgZ-1 has the ability to donate electrons to extracellular Fe(III) species. Inhibition tests revealed that dehydrogenases, quinones, and cytochromes b/c (cyt b/c) were involved in reduction of AQS (9, 10-anthraquinone-2-sulfonic acid, humus analog) and oxygen. In contrast, only NADH dehydrogenase was linked to electron transport to HFO, while dehydrogenases and cyt b/c were found to participate in the reduction of Fe-EDTA. Thus, various different electron transport pathways are employed by strain SgZ-1 for different electron acceptors. The results from this study help in understanding the electron transport processes and environmental responses of the genus Thauera. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2014 | ||
| 690 | 7 | |a Thauera |2 nationallicence | |
| 690 | 7 | |a Humus |2 nationallicence | |
| 690 | 7 | |a Fe(III) species |2 nationallicence | |
| 690 | 7 | |a Electron transport pathway |2 nationallicence | |
| 690 | 7 | |a c -type cytochrome |2 nationallicence | |
| 700 | 1 | |a Ma |D Chen |u Guangdong Institute of Eco-Environmental and Soil Sciences, 510650, Guangzhou, People's Republic of China |4 aut | |
| 700 | 1 | |a Yu |D Zhen |u Guangdong Institute of Eco-Environmental and Soil Sciences, 510650, Guangzhou, People's Republic of China |4 aut | |
| 700 | 1 | |a Lu |D Qin |u Guangdong Institute of Eco-Environmental and Soil Sciences, 510650, Guangzhou, People's Republic of China |4 aut | |
| 700 | 1 | |a Zhuang |D Li |u Guangdong Institute of Eco-Environmental and Soil Sciences, 510650, Guangzhou, People's Republic of China |4 aut | |
| 700 | 1 | |a Zhou |D Shun-Gui |u Guangdong Institute of Eco-Environmental and Soil Sciences, 510650, Guangzhou, People's Republic of China |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/8(2015-04-01), 3619-3628 |x 0175-7598 |q 99:8<3619 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-014-6254-x |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-014-6254-x |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ma |D Chen |u Guangdong Institute of Eco-Environmental and Soil Sciences, 510650, Guangzhou, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Yu |D Zhen |u Guangdong Institute of Eco-Environmental and Soil Sciences, 510650, Guangzhou, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Lu |D Qin |u Guangdong Institute of Eco-Environmental and Soil Sciences, 510650, Guangzhou, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhuang |D Li |u Guangdong Institute of Eco-Environmental and Soil Sciences, 510650, Guangzhou, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zhou |D Shun-Gui |u Guangdong Institute of Eco-Environmental and Soil Sciences, 510650, Guangzhou, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/8(2015-04-01), 3619-3628 |x 0175-7598 |q 99:8<3619 |1 2015 |2 99 |o 253 | ||