caa a22 4500 605504202 CHVBK 20210128100614.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s00253-014-6254-x doi (NATIONALLICENCE)springer-10.1007/s00253-014-6254-x Anaerobic humus and Fe(III) reduction and electron transport pathway by a novel humus-reducing bacterium, Thauera humireducens SgZ-1 [Elektronische Daten] [Chen Ma, Zhen Yu, Qin Lu, Li Zhuang, Shun-Gui Zhou] In this study, an anaerobic batch experiment was conducted to investigate the humus- and Fe(III)-reducing ability of a novel humus-reducing bacterium, Thauera humireducens SgZ-1. Inhibition tests were also performed to explore the electron transport pathways with various electron acceptors. The results indicate that in anaerobic conditions, strain SgZ-1 possesses the ability to reduce a humus analog, humic acids, soluble Fe(III), and Fe(III) oxides. Acetate, propionate, lactate, and pyruvate were suitable electron donors for humus and Fe(III) reduction by strain SgZ-1, while fermentable sugars (glucose and sucrose) were not. UV-visible spectra obtained from intact cells of strain SgZ-1 showed absorption peaks at 420, 522, and 553nm, characteristic of c-type cytochromes (cyt c). Dithionite-reduced cyt c was reoxidized by Fe-EDTA and HFO (hydrous ferric oxide), which suggests that cyt c within intact cells of strain SgZ-1 has the ability to donate electrons to extracellular Fe(III) species. Inhibition tests revealed that dehydrogenases, quinones, and cytochromes b/c (cyt b/c) were involved in reduction of AQS (9, 10-anthraquinone-2-sulfonic acid, humus analog) and oxygen. In contrast, only NADH dehydrogenase was linked to electron transport to HFO, while dehydrogenases and cyt b/c were found to participate in the reduction of Fe-EDTA. Thus, various different electron transport pathways are employed by strain SgZ-1 for different electron acceptors. The results from this study help in understanding the electron transport processes and environmental responses of the genus Thauera. Springer-Verlag Berlin Heidelberg, 2014 Thauera nationallicence Humus nationallicence Fe(III) species nationallicence Electron transport pathway nationallicence c -type cytochrome nationallicence Ma Chen Guangdong Institute of Eco-Environmental and Soil Sciences, 510650, Guangzhou, People's Republic of China aut Yu Zhen Guangdong Institute of Eco-Environmental and Soil Sciences, 510650, Guangzhou, People's Republic of China aut Lu Qin Guangdong Institute of Eco-Environmental and Soil Sciences, 510650, Guangzhou, People's Republic of China aut Zhuang Li Guangdong Institute of Eco-Environmental and Soil Sciences, 510650, Guangzhou, People's Republic of China aut Zhou Shun-Gui Guangdong Institute of Eco-Environmental and Soil Sciences, 510650, Guangzhou, People's Republic of China aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/8(2015-04-01), 3619-3628 0175-7598 99:8<3619 2015 99 253 https://doi.org/10.1007/s00253-014-6254-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6254-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ma Chen Guangdong Institute of Eco-Environmental and Soil Sciences, 510650, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Yu Zhen Guangdong Institute of Eco-Environmental and Soil Sciences, 510650, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Lu Qin Guangdong Institute of Eco-Environmental and Soil Sciences, 510650, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Zhuang Li Guangdong Institute of Eco-Environmental and Soil Sciences, 510650, Guangzhou, People's Republic of China aut NATIONALLICENCE 700 1- Zhou Shun-Gui Guangdong Institute of Eco-Environmental and Soil Sciences, 510650, Guangzhou, People's Republic of China aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/8(2015-04-01), 3619-3628 0175-7598 99:8<3619 2015 99 253