caa a22 4500 605504210 CHVBK 20210128100615.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s00253-014-6211-8 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6211-8 The concentration-determined and population-specific antimicrobial effects of free nitrous acid on Pseudomonas aeruginosa PAO1 [Elektronische Daten] [Shu-Hong Gao, Lu Fan, Zhiguo Yuan, Philip Bond] There is great potential to use free nitrous acid (FNA/HNO2), the protonated form of nitrite, as an antimicrobial agent due to its bacteriostatic and bactericidal effects on a range of microorganisms. Here, we determine the effects of FNA on the opportunistic pathogen Pseudomonas aeruginosa PAO1, a well-studied denitrifier capable of nitrate/nitrite reduction in its anaerobic respiration. It was seen that lower FNA concentrations in the range of 0.1 to 0.2mgN/L exerted a temporary inhibitory effect on the growth of P. aeruginosa, while respiratory inhibition was not detected until an FNA concentration of 1.0mgN/L was applied. The FNA concentration of 5.0mgN/L caused complete cell killing and likely cell lysis. The results suggest concentration-related and multiple antimicrobial effects of FNA. Differential killing of FNA in the P. aeruginosa subpopulations was detected, suggesting intrastrain heterogeneity, and does not support the idea of specific concentrations of FNA bringing about bacteriostatic and bactericidal effects on this species. A delayed recovery from FNA treatment suggested that FNA caused cell damage which required repair prior to the organism showing cell growth. The results of the study provide insight of the inhibitory and biocidal mechanisms of FNA on this important microorganism. Springer-Verlag Berlin Heidelberg, 2014 Free nitrous acid nationallicence Bacteriostatic nationallicence Bactericidal nationallicence Pseudomonas aeruginosa PAO1 nationallicence Gao Shu-Hong Advanced Water Management Centre, The University of Queensland, Level 4 Gehrmann Building, St. Lucia, 4072, Brisbane, QLD, Australia aut Fan Lu Advanced Water Management Centre, The University of Queensland, Level 4 Gehrmann Building, St. Lucia, 4072, Brisbane, QLD, Australia aut Yuan Zhiguo Advanced Water Management Centre, The University of Queensland, Level 4 Gehrmann Building, St. Lucia, 4072, Brisbane, QLD, Australia aut Bond Philip Advanced Water Management Centre, The University of Queensland, Level 4 Gehrmann Building, St. Lucia, 4072, Brisbane, QLD, Australia aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/5(2015-03-01), 2305-2312 0175-7598 99:5<2305 2015 99 253 https://doi.org/10.1007/s00253-014-6211-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6211-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gao Shu-Hong Advanced Water Management Centre, The University of Queensland, Level 4 Gehrmann Building, St. Lucia, 4072, Brisbane, QLD, Australia aut NATIONALLICENCE 700 1- Fan Lu Advanced Water Management Centre, The University of Queensland, Level 4 Gehrmann Building, St. Lucia, 4072, Brisbane, QLD, Australia aut NATIONALLICENCE 700 1- Yuan Zhiguo Advanced Water Management Centre, The University of Queensland, Level 4 Gehrmann Building, St. Lucia, 4072, Brisbane, QLD, Australia aut NATIONALLICENCE 700 1- Bond Philip Advanced Water Management Centre, The University of Queensland, Level 4 Gehrmann Building, St. Lucia, 4072, Brisbane, QLD, Australia aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/5(2015-03-01), 2305-2312 0175-7598 99:5<2305 2015 99 253