caa a22 4500 605504229 CHVBK 20210128100615.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s00253-014-6325-z doi (NATIONALLICENCE)springer-10.1007/s00253-014-6325-z Fundamental role of cobalamin biosynthesis in the developmental growth of Streptomyces coelicolor A3 (2) [Elektronische Daten] [Hideaki Takano, Kenta Hagiwara, Kenji Ueda] Cobalamin (Cbl) (synonym, vitamin B12) is the cobalt-containing cofactor produced only by some prokaryotes. Streptomyces is an effective Cbl producer. To study the role of Cbl production in Streptomyces, a knockout mutant for Cbl biosynthesis (cob) was generated in Streptomyces coelicolor A3 (2). The growth of the mutant was similar to that of the wild type in a rich medium, but inhibited in minimal medium, suggesting the involvement of Cbl in some step of primary metabolism. Methionine synthesis catalyzed by MetH, the Cbl-dependent methionine synthase, is a candidate. However, supplementing the minimal medium with methionine did not rescue the growth of the cob mutant, indicating that the availability of Cbl affects another primary function. Transcriptional analysis confirmed that the mutant induced metE encoding an alternative Cbl-independent methionine synthase, probably due to the Cbl-dependent riboswitch mechanism. The cob mutant produced low levels of pigment antibiotics and formed fewer aerial mycelium and spores in a rich medium, suggesting that a Cbl-dependent mechanism controls development. A similar developmental defect was observed for a knockout mutant for SCO4800, encoding the putative Cbl-dependent isobutyryl-CoA mutase (Icm) small subunit. Since the knockout of the Icm large subunit (SCO5415) did not affect the developmental phenotype, SCO4800 likely regulates development independently from SCO5415. Effective Cbl production is fundamental to the diverse functions underlying the complex developmental life cycle of S. coelicolor A3 (2). Springer-Verlag Berlin Heidelberg, 2014 Cobalamin nationallicence Streptomyces nationallicence Antibiotic production nationallicence Cell differentiation nationallicence Riboswitch nationallicence Takano Hideaki Life Science Research Center, College of Bioresource Sciences, Nihon University, 1866 Kameino, 252-0880, Fujisawa, Japan aut Hagiwara Kenta Life Science Research Center, College of Bioresource Sciences, Nihon University, 1866 Kameino, 252-0880, Fujisawa, Japan aut Ueda Kenji Life Science Research Center, College of Bioresource Sciences, Nihon University, 1866 Kameino, 252-0880, Fujisawa, Japan aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/5(2015-03-01), 2329-2337 0175-7598 99:5<2329 2015 99 253 https://doi.org/10.1007/s00253-014-6325-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6325-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Takano Hideaki Life Science Research Center, College of Bioresource Sciences, Nihon University, 1866 Kameino, 252-0880, Fujisawa, Japan aut NATIONALLICENCE 700 1- Hagiwara Kenta Life Science Research Center, College of Bioresource Sciences, Nihon University, 1866 Kameino, 252-0880, Fujisawa, Japan aut NATIONALLICENCE 700 1- Ueda Kenji Life Science Research Center, College of Bioresource Sciences, Nihon University, 1866 Kameino, 252-0880, Fujisawa, Japan aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/5(2015-03-01), 2329-2337 0175-7598 99:5<2329 2015 99 253