Upscale production of ethyl ( S )-4-chloro-3-hydroxybutanoate by using carbonyl reductase coupled with glucose dehydrogenase in aqueous-organic solvent system
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| 024 | 7 | 0 | |a 10.1007/s00253-014-6245-y |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-014-6245-y | ||
| 245 | 0 | 0 | |a Upscale production of ethyl ( S )-4-chloro-3-hydroxybutanoate by using carbonyl reductase coupled with glucose dehydrogenase in aqueous-organic solvent system |h [Elektronische Daten] |c [Zhi-Qiang Liu, Jing-Jing Ye, Zhen-Yang Shen, Hua-Bin Hong, Jian-Bo Yan, Yi Lin, Zheng-Xu Chen, Yu-Guo Zheng, Yin-Chu Shen] |
| 520 | 3 | |a (S)-4-chloro-3-hydroxybutanoate ((S)-CHBE) is an important chiral intermediate to synthesize the side chain of cholesterol-lowering drug atorvastatin. To biosynthesize the (S)-CHBE, a recombinant Escherichia coli harboring the carbonyl reductase and glucose dehydrogenase was successfully constructed. The recombinant E. coli was cultured in a 500-L fermentor; after induction and expression, the enzyme activity and cell biomass were increased to 23,661.65U/L and 13.90g DCW/L which was 3.24 and 2.60-folds compared with those in the 50L fermentor. The biocatalytic process for the synthesis of (S)-CHBE in an aqueous-organic solvent system was constructed and optimized with a substrate fed-batch strategy. The ethyl 4-chloro-3-oxobutanoate concentration reached to 1.7M, and the (S)-CHBE with yield of 97.2% and enantiomeric excess (e.e.) of 99% was obtained after 4-h reaction in a 50-L reactor. In this study, the space-time yield and space-time yield per gram of biomass (dry cell weight, DCW) were 413.17mM/h and 27.55mM/h/g DCWfor(S)-CHBE production, respectively, which were the highest values as compared to previous reports. Finally, (S)-CHBE was extracted from the reaction mixture with 82% of yield and 95% of purity. This study paved the foundation for the upscale production of (S)-CHBE by biocatalysis method. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2014 | ||
| 690 | 7 | |a Co-expression |2 nationallicence | |
| 690 | 7 | |a Carbonyl reductase |2 nationallicence | |
| 690 | 7 | |a Glucose dehydrogenase |2 nationallicence | |
| 690 | 7 | |a Ethyl ( S )-4-chloro-3-hydroxybutanoate |2 nationallicence | |
| 700 | 1 | |a Liu |D Zhi-Qiang |u Institute of Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, Zhejiang, People's Republic of China |4 aut | |
| 700 | 1 | |a Ye |D Jing-Jing |u Institute of Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, Zhejiang, People's Republic of China |4 aut | |
| 700 | 1 | |a Shen |D Zhen-Yang |u Institute of Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, Zhejiang, People's Republic of China |4 aut | |
| 700 | 1 | |a Hong |D Hua-Bin |u Zhejiang Neo-DanKong Pharmaceutical Co., Ltd, 318000, Taizhou, Zhejiang, People's Republic of China |4 aut | |
| 700 | 1 | |a Yan |D Jian-Bo |u Zhejiang Neo-DanKong Pharmaceutical Co., Ltd, 318000, Taizhou, Zhejiang, People's Republic of China |4 aut | |
| 700 | 1 | |a Lin |D Yi |u Zhejiang Neo-DanKong Pharmaceutical Co., Ltd, 318000, Taizhou, Zhejiang, People's Republic of China |4 aut | |
| 700 | 1 | |a Chen |D Zheng-Xu |u Zhejiang Neo-DanKong Pharmaceutical Co., Ltd, 318000, Taizhou, Zhejiang, People's Republic of China |4 aut | |
| 700 | 1 | |a Zheng |D Yu-Guo |u Institute of Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, Zhejiang, People's Republic of China |4 aut | |
| 700 | 1 | |a Shen |D Yin-Chu |u Institute of Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, Zhejiang, People's Republic of China |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/5(2015-03-01), 2119-2129 |x 0175-7598 |q 99:5<2119 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-014-6245-y |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a research-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-014-6245-y |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Liu |D Zhi-Qiang |u Institute of Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, Zhejiang, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Ye |D Jing-Jing |u Institute of Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, Zhejiang, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Shen |D Zhen-Yang |u Institute of Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, Zhejiang, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Hong |D Hua-Bin |u Zhejiang Neo-DanKong Pharmaceutical Co., Ltd, 318000, Taizhou, Zhejiang, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Yan |D Jian-Bo |u Zhejiang Neo-DanKong Pharmaceutical Co., Ltd, 318000, Taizhou, Zhejiang, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Lin |D Yi |u Zhejiang Neo-DanKong Pharmaceutical Co., Ltd, 318000, Taizhou, Zhejiang, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Chen |D Zheng-Xu |u Zhejiang Neo-DanKong Pharmaceutical Co., Ltd, 318000, Taizhou, Zhejiang, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Zheng |D Yu-Guo |u Institute of Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, Zhejiang, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Shen |D Yin-Chu |u Institute of Bioengineering, Zhejiang University of Technology, 310014, Hangzhou, Zhejiang, People's Republic of China |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/5(2015-03-01), 2119-2129 |x 0175-7598 |q 99:5<2119 |1 2015 |2 99 |o 253 | ||