caa a22 4500 605504334 CHVBK 20210128100615.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s00253-014-6127-3 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6127-3 Effects of selenium oxyanions on the white-rot fungus Phanerochaete chrysosporium [Elektronische Daten] [Erika Espinosa-Ortiz, Graciela Gonzalez-Gil, Pascal Saikaly, Eric van Hullebusch, Piet Lens] The ability of Phanerochaete chrysosporium to reduce the oxidized forms of selenium, selenate and selenite, and their effects on the growth, substrate consumption rate, and pellet morphology of the fungus were assessed. The effect of different operational parameters (pH, glucose, and selenium concentration) on the response of P. chrysosporium to selenium oxyanions was explored as well. This fungal species showed a high sensitivity to selenium, particularly selenite, which inhibited the fungal growth and substrate consumption when supplied at 10mgL−1 in the growth medium, whereas selenate did not have such a strong influence on the fungus. Biological removal of selenite was achieved under semi-acidic conditions (pH 4.5) with about 40% removal efficiency, whereas less than 10% selenium removal was achieved for incubations with selenate. P. chrysosporium was found to be a selenium-reducing organism, capable of synthesizing elemental selenium from selenite but not from selenate. Analysis with transmission electron microscopy, electron energy loss spectroscopy, and a 3D reconstruction showed that elemental selenium was produced intracellularly as nanoparticles in the range of 30-400nm. Furthermore, selenite influenced the pellet morphology of P. chrysosporium by reducing the size of the fungal pellets and inducing their compaction and smoothness. Springer-Verlag Berlin Heidelberg, 2014 Fungal pellets nationallicence Selenium removal nationallicence Selenium nanoparticles nationallicence Phanerochaete chrysosporium nationallicence Espinosa-Ortiz Erika UNESCO-IHE Institute for Water Education, P.O. Box 3015, 2601 DA, Delft, The Netherlands aut Gonzalez-Gil Graciela UNESCO-IHE Institute for Water Education, P.O. Box 3015, 2601 DA, Delft, The Netherlands aut Saikaly Pascal Water Desalination and Reuse Center, Division of Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology, 13955-69000, Thuwal, Saudi Arabia aut van Hullebusch Eric Laboratoire Géomatériaux et Environnement (EA 4508), UPEM, Université Paris-Est, 77454, Marne-la-Vallée, France aut Lens Piet UNESCO-IHE Institute for Water Education, P.O. Box 3015, 2601 DA, Delft, The Netherlands aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/5(2015-03-01), 2405-2418 0175-7598 99:5<2405 2015 99 253 https://doi.org/10.1007/s00253-014-6127-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6127-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Espinosa-Ortiz Erika UNESCO-IHE Institute for Water Education, P.O. Box 3015, 2601 DA, Delft, The Netherlands aut NATIONALLICENCE 700 1- Gonzalez-Gil Graciela UNESCO-IHE Institute for Water Education, P.O. Box 3015, 2601 DA, Delft, The Netherlands aut NATIONALLICENCE 700 1- Saikaly Pascal Water Desalination and Reuse Center, Division of Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology, 13955-69000, Thuwal, Saudi Arabia aut NATIONALLICENCE 700 1- van Hullebusch Eric Laboratoire Géomatériaux et Environnement (EA 4508), UPEM, Université Paris-Est, 77454, Marne-la-Vallée, France aut NATIONALLICENCE 700 1- Lens Piet UNESCO-IHE Institute for Water Education, P.O. Box 3015, 2601 DA, Delft, The Netherlands aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/5(2015-03-01), 2405-2418 0175-7598 99:5<2405 2015 99 253