caa a22 4500 605504342 CHVBK 20210128100615.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s00253-014-6159-8 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6159-8 Importance of Rhodococcus strains in a bacterial consortium degrading a mixture of hydrocarbons, gasoline, and diesel oil additives revealed by metatranscriptomic analysis [Elektronische Daten] [Marc Auffret, Etienne Yergeau, Diane Labbé, Françoise Fayolle-Guichard, Charles Greer] A bacterial consortium (Mix3) composed of microorganisms originating from different environments (soils and wastewater) was obtained after enrichment in the presence of a mixture of 16 hydrocarbons, gasoline, and diesel oil additives. After addition of the mixture, the development of the microbial composition of Mix3 was monitored at three different times (35, 113, and 222days) using fingerprinting method and dominant bacterial species were identified. In parallel, 14 bacteria were isolated after 113days and identified. Degradation capacities for Mix3 and the isolated bacterial strains were characterized and compared. At day 113, we induced the expression of catabolic genes in Mix3 by adding the substrate mixture to resting cells and the metatranscriptome was analyzed. After addition of the substrate mixture, the relative abundance of Actinobacteria increased at day 222 while a shift between Rhodococcus and Mycobacterium was observed after 113days. Mix3 was able to degrade 13 compounds completely, with partial degradation of isooctane and 2-ethylhexyl nitrate, but tert-butyl alcohol was not degraded. Rhodococcus wratislaviensis strain IFP 2016 isolated from Mix3 showed almost the same degradation capacities as Mix3: these results were not observed with the other isolated strains. Transcriptomic results revealed that Actinobacteria and in particular, Rhodococcus species, were major contributors in terms of total and catabolic gene transcripts while other species were involved in cyclohexane degradation. Not all the microorganisms identified at day 113 were active except R. wratislaviensis IFP 2016 that appeared to be a major player in the degradation activity observed in Mix3. Springer-Verlag Berlin Heidelberg, 2014 Rhodococcus nationallicence Metatranscriptomic analysis nationallicence Biodegradation activities nationallicence Dynamics of gene expression nationallicence Bacterial enrichment nationallicence Auffret Marc Institut Français du Pétrole (IFP), 1-4 Avenue de Bois-Préau, 92852, Rueil-Malmaison, France aut Yergeau Etienne National Research Council Canada (NRC), 6100 Royalmount Avenue, H4P 2R2, Montréal, Québec, Canada aut Labbé Diane National Research Council Canada (NRC), 6100 Royalmount Avenue, H4P 2R2, Montréal, Québec, Canada aut Fayolle-Guichard Françoise Institut Français du Pétrole (IFP), 1-4 Avenue de Bois-Préau, 92852, Rueil-Malmaison, France aut Greer Charles National Research Council Canada (NRC), 6100 Royalmount Avenue, H4P 2R2, Montréal, Québec, Canada aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/5(2015-03-01), 2419-2430 0175-7598 99:5<2419 2015 99 253 https://doi.org/10.1007/s00253-014-6159-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6159-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Auffret Marc Institut Français du Pétrole (IFP), 1-4 Avenue de Bois-Préau, 92852, Rueil-Malmaison, France aut NATIONALLICENCE 700 1- Yergeau Etienne National Research Council Canada (NRC), 6100 Royalmount Avenue, H4P 2R2, Montréal, Québec, Canada aut NATIONALLICENCE 700 1- Labbé Diane National Research Council Canada (NRC), 6100 Royalmount Avenue, H4P 2R2, Montréal, Québec, Canada aut NATIONALLICENCE 700 1- Fayolle-Guichard Françoise Institut Français du Pétrole (IFP), 1-4 Avenue de Bois-Préau, 92852, Rueil-Malmaison, France aut NATIONALLICENCE 700 1- Greer Charles National Research Council Canada (NRC), 6100 Royalmount Avenue, H4P 2R2, Montréal, Québec, Canada aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/5(2015-03-01), 2419-2430 0175-7598 99:5<2419 2015 99 253