caa a22 4500 605504369 CHVBK 20210128100615.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s00253-014-6140-6 doi (NATIONALLICENCE)springer-10.1007/s00253-014-6140-6 Effect of S/N ratio on sulfide removal by autotrophic denitrification [Elektronische Daten] [Petr Dolejs, Ladislav Paclík, Josef Maca, Dana Pokorna, Jana Zabranska, Jan Bartacek] In this study a completely stirred tank reactor was used to study the effect of sulfide to nitrate (S/N) ratio on sulfide removal while nitrate was used as electron acceptor. Several S/N ratios were studied for this purpose ranging from 0.3 to 2.4mol/mol. The complete sulfide removal was achieved when S/N ratio 0.85mol/mol was used with the autotrophic denitrification efficiency up to 80%. No nitrite accumulation was observed, and the main product of sulfide oxidation was sulfate. Dissimilatory nitrogen reduction to ammonia occurred and subsequently, elemental sulfur accumulated while S/N ratio was higher than 1.3mol/mol. The specific autotrophic denitrification rates under S/N ratios 0.8 and 1.2 were 5 and 26mgg−1h−1 (N-NO3 −, VSS), respectively. Thiobacillus denitrificans and Thiomicrospira denitrificans were detected in the reactor by fluorescent in situ hybridization, but their overall representation was not more than 5% of the entire microbial populations. Springer-Verlag Berlin Heidelberg, 2014 Autotrophic denitrification nationallicence Completely stirred tank reactor (CSTR) nationallicence S/N ratio nationallicence Sulfide removal nationallicence Thiobacillus denitrificans nationallicence Thiomicrospira denitrificans nationallicence Dolejs Petr Department of Water Technology and Environmental Engineering, Faculty of Environmental Technology, Institute of Chemical Technology, Technická 5, 166 28, Prague 6, The Czech Republic aut Paclík Ladislav Department of Water Technology and Environmental Engineering, Faculty of Environmental Technology, Institute of Chemical Technology, Technická 5, 166 28, Prague 6, The Czech Republic aut Maca Josef Department of Water Technology and Environmental Engineering, Faculty of Environmental Technology, Institute of Chemical Technology, Technická 5, 166 28, Prague 6, The Czech Republic aut Pokorna Dana Department of Water Technology and Environmental Engineering, Faculty of Environmental Technology, Institute of Chemical Technology, Technická 5, 166 28, Prague 6, The Czech Republic aut Zabranska Jana Department of Water Technology and Environmental Engineering, Faculty of Environmental Technology, Institute of Chemical Technology, Technická 5, 166 28, Prague 6, The Czech Republic aut Bartacek Jan Department of Water Technology and Environmental Engineering, Faculty of Environmental Technology, Institute of Chemical Technology, Technická 5, 166 28, Prague 6, The Czech Republic aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/5(2015-03-01), 2383-2392 0175-7598 99:5<2383 2015 99 253 https://doi.org/10.1007/s00253-014-6140-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-014-6140-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dolejs Petr Department of Water Technology and Environmental Engineering, Faculty of Environmental Technology, Institute of Chemical Technology, Technická 5, 166 28, Prague 6, The Czech Republic aut NATIONALLICENCE 700 1- Paclík Ladislav Department of Water Technology and Environmental Engineering, Faculty of Environmental Technology, Institute of Chemical Technology, Technická 5, 166 28, Prague 6, The Czech Republic aut NATIONALLICENCE 700 1- Maca Josef Department of Water Technology and Environmental Engineering, Faculty of Environmental Technology, Institute of Chemical Technology, Technická 5, 166 28, Prague 6, The Czech Republic aut NATIONALLICENCE 700 1- Pokorna Dana Department of Water Technology and Environmental Engineering, Faculty of Environmental Technology, Institute of Chemical Technology, Technická 5, 166 28, Prague 6, The Czech Republic aut NATIONALLICENCE 700 1- Zabranska Jana Department of Water Technology and Environmental Engineering, Faculty of Environmental Technology, Institute of Chemical Technology, Technická 5, 166 28, Prague 6, The Czech Republic aut NATIONALLICENCE 700 1- Bartacek Jan Department of Water Technology and Environmental Engineering, Faculty of Environmental Technology, Institute of Chemical Technology, Technická 5, 166 28, Prague 6, The Czech Republic aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/5(2015-03-01), 2383-2392 0175-7598 99:5<2383 2015 99 253