Combinatorial and high-throughput screening approaches for strain engineering
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| 024 | 7 | 0 | |a 10.1007/s00253-015-6400-0 |2 doi |
| 035 | |a (NATIONALLICENCE)springer-10.1007/s00253-015-6400-0 | ||
| 245 | 0 | 0 | |a Combinatorial and high-throughput screening approaches for strain engineering |h [Elektronische Daten] |c [Wenshan Liu, Rongrong Jiang] |
| 520 | 3 | |a Microbes have long been used in the industry to produce valuable biochemicals. Combinatorial engineering approaches, new strain engineering tools derived from inverse metabolic engineering, have started to attract attention in recent years, including genome shuffling, error-prone DNA polymerase, global transcription machinery engineering (gTME), random knockout/overexpression libraries, ribosome engineering, multiplex automated genome engineering (MAGE), customized optimization of metabolic pathways by combinatorial transcriptional engineering (COMPACTER), and library construction of "tunable intergenic regions” (TIGR). Since combinatorial approaches and high-throughput screening methods are fundamentally interconnected, color/fluorescence-based, growth-based, and biosensor-based high-throughput screening methods have been reviewed. We believe that with the help of metabolic engineering tools and new combinatorial approaches, plus effective high-throughput screening methods, researchers will be able to achieve better results on improving microorganism performance under stress or enhancing biochemical yield. | |
| 540 | |a Springer-Verlag Berlin Heidelberg, 2015 | ||
| 690 | 7 | |a Strain engineering |2 nationallicence | |
| 690 | 7 | |a Combinatorial engineering |2 nationallicence | |
| 690 | 7 | |a Transcriptional engineering |2 nationallicence | |
| 690 | 7 | |a High-throughput screening |2 nationallicence | |
| 690 | 7 | |a Global transcription machinery engineering |2 nationallicence | |
| 690 | 7 | |a Multiplex automated genome engineering |2 nationallicence | |
| 690 | 7 | |a Fine tuning of gene expression |2 nationallicence | |
| 700 | 1 | |a Liu |D Wenshan |u School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, 637459, Singapore, Singapore |4 aut | |
| 700 | 1 | |a Jiang |D Rongrong |u School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, 637459, Singapore, Singapore |4 aut | |
| 773 | 0 | |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/5(2015-03-01), 2093-2104 |x 0175-7598 |q 99:5<2093 |1 2015 |2 99 |o 253 | |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00253-015-6400-0 |q text/html |z Onlinezugriff via DOI |
| 898 | |a BK010053 |b XK010053 |c XK010000 | ||
| 900 | 7 | |a Metadata rights reserved |b Springer special CC-BY-NC licence |2 nationallicence | |
| 908 | |D 1 |a review-article |2 jats | ||
| 949 | |B NATIONALLICENCE |F NATIONALLICENCE |b NL-springer | ||
| 950 | |B NATIONALLICENCE |P 856 |E 40 |u https://doi.org/10.1007/s00253-015-6400-0 |q text/html |z Onlinezugriff via DOI | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Liu |D Wenshan |u School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, 637459, Singapore, Singapore |4 aut | ||
| 950 | |B NATIONALLICENCE |P 700 |E 1- |a Jiang |D Rongrong |u School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, 637459, Singapore, Singapore |4 aut | ||
| 950 | |B NATIONALLICENCE |P 773 |E 0- |t Applied Microbiology and Biotechnology |d Springer Berlin Heidelberg |g 99/5(2015-03-01), 2093-2104 |x 0175-7598 |q 99:5<2093 |1 2015 |2 99 |o 253 | ||