caa a22 4500 605504385 CHVBK 20210128100615.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s00253-015-6400-0 doi (NATIONALLICENCE)springer-10.1007/s00253-015-6400-0 Combinatorial and high-throughput screening approaches for strain engineering [Elektronische Daten] [Wenshan Liu, Rongrong Jiang] Microbes have long been used in the industry to produce valuable biochemicals. Combinatorial engineering approaches, new strain engineering tools derived from inverse metabolic engineering, have started to attract attention in recent years, including genome shuffling, error-prone DNA polymerase, global transcription machinery engineering (gTME), random knockout/overexpression libraries, ribosome engineering, multiplex automated genome engineering (MAGE), customized optimization of metabolic pathways by combinatorial transcriptional engineering (COMPACTER), and library construction of "tunable intergenic regions” (TIGR). Since combinatorial approaches and high-throughput screening methods are fundamentally interconnected, color/fluorescence-based, growth-based, and biosensor-based high-throughput screening methods have been reviewed. We believe that with the help of metabolic engineering tools and new combinatorial approaches, plus effective high-throughput screening methods, researchers will be able to achieve better results on improving microorganism performance under stress or enhancing biochemical yield. Springer-Verlag Berlin Heidelberg, 2015 Strain engineering nationallicence Combinatorial engineering nationallicence Transcriptional engineering nationallicence High-throughput screening nationallicence Global transcription machinery engineering nationallicence Multiplex automated genome engineering nationallicence Fine tuning of gene expression nationallicence Liu Wenshan School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, 637459, Singapore, Singapore aut Jiang Rongrong School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, 637459, Singapore, Singapore aut Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/5(2015-03-01), 2093-2104 0175-7598 99:5<2093 2015 99 253 https://doi.org/10.1007/s00253-015-6400-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 review-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00253-015-6400-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Wenshan School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, 637459, Singapore, Singapore aut NATIONALLICENCE 700 1- Jiang Rongrong School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, 637459, Singapore, Singapore aut NATIONALLICENCE 773 0- Applied Microbiology and Biotechnology Springer Berlin Heidelberg 99/5(2015-03-01), 2093-2104 0175-7598 99:5<2093 2015 99 253